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| Antigenic Specificity | ATM pS1981 |
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| Clone | 10H11.E12 |
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| Host Species | Mouse |
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| Reactive Species | human, mouse, rat |
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| Isotype | IgG1 kappa |
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| Format | unconjugated |
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| Size | 100 µg |
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| Concentration | 1.0 mg/mL |
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| Applications | ELISA, Immunohistochemistry, IF Microscopy, Western Blot. Protein A Purified Mab anti-ATM has been tested by ELISA and western blotting against both the native and recombinant forms of the protein. The antibody immunoprecipitates ATM from irradiated human and transfected mouse cells. By immunofluoresence, foci are detected in irradiated human and mouse fibroblasts. This antibody is not recommended for immunohistochemistry. Instead, for IHC, use the clone 7C10D8 (p/n 200-301-500). |
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| Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
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| Description | Anti-ATM phospho S1981 Monoclonal Antibody antibody is directed against human ATM and is useful in determining its presence in various assays. This monoclonal anti-ATM antibody recognizes the phosphorylated epitope in native and over-expressed proteins found in various tissues and extracts. By ELISA reactivity against SLAFEEGSpQSTTISS at a 1:1600 dilution shows an absorbance >3.000; whereas reactivity against SLAFEEGSQSTTISS shows and absorbance of 0.145. Reactivity is observed against human ATM. Cross reactivity with ATM from other mammalian sources has not been tested. The immunogen has 91% sequence homology with mouse ATM. |
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| Immunogen | Anti-ATM phospho S1981 Antibody was produced from a synthetic peptide S-L-A-F-E-E-G-Sp-Q-S-T-T-I-S-S corresponding to aa 1974-1988 of human ATM. |
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| Other Names | mouse anti-ATM antibody, mouse anti-ATMpS1981 antibody, mouse anti- ATM pS1981 antibody, DKFZp781A0353 antibody, Human phosphatidylinositol 3 kinase homolog antibody, MGC74674 antibody, Serine protein kinase ATM antibody, T cell prolymphocytic leukemia antibody |
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| Gene, Accession # | ATM, Gene ID: 472, NCBI: Q13315.3, UniProt: Q13315 |
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| Catalog # | 200-301-400 |
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| Price | |
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| Order / More Info | ATM pS1981 Antibody from ROCKLAND IMMUNOCHEMICALS INC. |
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| Product Specific References | Bakkenist, C. J. & Kastan, M. B. (2003). DNA damage activates ATM through intermolecular autophosphorylation and dimer dissociation. Nature 421, 499-506.Kitagawa R, Bakkenist CJ, McKinnon PJ, Kastan MB. (2004) Phosphorylation of SMC1 is a critical downstream event in the ATM-NBS1-BRCA1 pathway. Genes Dev. 18(12):1423-38.Falck, J. Coates, J. and Jackson, S.P. (2005) Conserved modes of recruitment of ATM, ATR and DNA-PKcs to sites of DNA damage. Nature 434: 605-611.Bartkova J, Horejsi Z, Koed K, Kramer A, Tort F, Zieger K, Guldberg P, Sehested M, Nesland JM, Lukas C, Orntoft T, Lukas J, Bartek J. (2005) DNA damage response as a candidate anti-cancer barrier in early human tumorigenesis. Nature 434; 864-870. Bartkova J, Bakkenist CJ, Rajpert-De Meyts E, Skakkebaek NE, Sehested M, Lukas J, Kastan MB, Bartek J. (2005) ATM Activation in Normal Human Tissues and Testicular Cancer. Cell Cycle 4;(6) [Epub ahead of print].Pusapati, R. V., Rounbehler, R. J., Hong, S., Powers, J. T., Yan, M., Kiguchi, K., ... & Johnson, D. G. (2006). ATM promotes apoptosis and suppresses tumorigenesis in response to Myc. Proceedings of the National Academy of Sciences of the United States of America, 103(5), 1446-1451Zhang, W., Peng, G., Lin, S. Y., & Zhang, P. (2011). DNA damage response is suppressed by the high cyclin-dependent kinase 1 activity in mitotic mammalian cells. Journal of Biological Chemistry, 286(41), 35899-35905. |
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