| 产品详情 |
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| Antigenic Specificity | ATG13 phospho S318, internal |
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| Clone | polyclonal |
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| Host Species | Rabbit |
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| Reactive Species | human |
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| Isotype | n/a |
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| Format | immunoaffinity purified |
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| Size | 100 µg |
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| Concentration | 1.1 mg/mL |
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| Applications | ELISA, Flow Cytometry, Western Blot. This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 56.6 kDa in size corresponding to human phosphorylated ATG13 protein by western blotting in the appropriate stimulated tissue or cell lysate or extract. |
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| Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
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| Description | This affinity-purified antibody is directed against the phosphorylated form of human ATG13 protein at the pS318 residue. The product was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross adsorbed against the non-phosphorylated form of the immunizing peptide. Reactivity occurs against human ATG13 pS318 protein and the antibody is specific for the phosphorylated form of the protein. Reactivity with non-phosphorylated human ATG13 is minimal by ELISA and western blot. A BLAST analysis was used to suggest cross reactivity with ATG13 from human based on 100% sequence homology with the immunogen. Reactivity against homologues from other sources is not known. |
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| Immunogen | Anti-ATG13 pS318 antibody was prepared by repeated immunizations with a synthetic peptide corresponding to the region near S318 of ATG13. |
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| Other Names | rabbit anti-ATG13 pS318 Antibody, ATG-13, ATG 13, Autophagy-related protein 13, KIAA0652 |
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| Gene, Accession # | ATG13, Gene ID: 9776, NCBI: NP_001136145.1, UniProt: O75143 |
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| Catalog # | 600-401-C49 |
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| Price | |
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| Order / More Info | ATG13 phospho S318, internal Antibody from ROCKLAND IMMUNOCHEMICALS INC. |
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| Product Specific References | Egan, D. F., Chun, M. G., Vamos, M., Zou, H., Rong, J., Miller, C. J., ... & Shaw, R. J. (2015). Small Molecule Inhibition of the Autophagy Kinase ULK1 and Identification of ULK1 Substrates. Molecular cell, 59(2), 285-297.Joo JH, Dorsey FC, Joshi A, Hennessy-Walters KM, Rose KL, McCastlain K, Zhang J, Iyengar R, Jung CH, Suen DF, Steeves MA, Yang CY, Prater SM, Kim DH, Thompson CB, Youle RJ, Ney PA, Cleveland JL, Kundu M. (2011) Hsp90-Cdc37 Chaperone Complex Regulates Ulk1- and Atg13-Mediated Mitophagy. Mol Cell. Aug 19;43 (4):572-85. Nazio F, Strappazzon F, Antonioli M, Bielli P, Cianfanelli V, Bordi M, Gretzmeier C, Dengjel J, Piacentini M, Fimia GM, Cecconi F. (2013) mTOR inhibits autophagy by controlling ULK1 ubiquitylation, self-association and function through AMBRA1 and TRAF6. Nat Cell Biol. Apr;15 (4):406-16.Stanton MJ, Dutta S, Zhang H, Polavaram NS, Leontovich AA, Hoenscheid P, Sinicrope FA, Tindall DJ, Muders MH, Datta K. (2013) Autophagy Control by the VEGF-C/NRP-2 Axis in Cancer and Its Implication for Treatment Resistance. Cancer Res. Jan 1;73 (1):160-71.Jiao H, Su GQ, Dong W, Zhang L, Xie W, Yao LM, Chen P, Wang ZX, Liou YC, You H. (2015) Chaperone-like protein p32 regulates ULK1 stability and autophagy. Cell Death Differ. 2015 Nov;22(11):1812-23. doi: 10.1038/cdd.2015.34. Epub 2015 Apr 23. |
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