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| Product Name | PEP, Colorimetric/Fluorometric Assay Kit, BioAssay™ (Phosphoenolpyruvate) |
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| Description | Phosphoenolpyruvate (PEP) is an important intermediate in carbohydrate metabolism. Containing a high-energy phosphate bond, PEP is involved in glycolysis and gluco-neogenesis and in the shikimate pathway as well as in carbon fixation in plants. Bacteria utilize PEP in the phosphor-transferase system to acquire sugars from the environment. In the glycolytic pathway, PEP is formed from 2-phosphoglycerate by enolase and generates ATP through the action of pyruvate kinase. USBio's PEP BioAssay™ Kit provides a convenient colorimetric and fluorometric means to measure PEP levels in various samples. In the assay, PEP is converted to ATP and pyruvate. The generated pyruvate is quantified by colorimetric (lmax = 570nm) or fluorometric methods (Ex/Em = 535/587nm). The assay is simple, sensitive and reliable. The detection limit is approximately 1uM PEP in biological samples. Sample Type: Cell/tissue lysates, urine, plasma, serum, and plant extracts. Intended Use: A convenient colorimetric |
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| Size | 100 tests |
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| Concentration | n/a |
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| Applications | n/a |
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| Other Names | n/a |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | P4072-54M |
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| Order / More Info | PEP, Colorimetric/Fluorometric Assay Kit, BioAssay™ (Phosphoenolpyruvate) from UNITED STATES BIOLOGICAL |
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| Product Specific References | n/a |
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