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| Product Name | Beta-Galactosidase Assay Kit (green fluorescence) |
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| Description | Amplite™ Fluorimetric Beta-Galactosidase Assay Kit *Green Fluorescence*. E. coli beta-galactosidase is a 464 kD tetramer. Each unit of beta-galactosidase consists of five domains, the third of which is the active site. It is an essential enzyme in cells. Deficiencies in this enzyme can result in galactosialidosis or Morquio B syndrome. In E. coli, beta-galactosidase is produced by the activation of LacZ operon. Detection of LacZ expression has become routine to the point of detection of as few as 5 copies of ?-galactosidase per cell. This kit uses a fluorogenic galactosidase substrate that can sensitively distinguish LacZ+ vs. LacZ-cells. It can be used either for detecting galactosidase conjugates in ELISA type assay systems or for monitoring LacZ gene expression in cells. The galactosidase-cleaved product has Ex/Em = 490/520 nm that can be detected with most of fluorescence instruments equipped with a FITC filter set. |
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| Size | 500 tests |
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| Concentration | n/a |
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| Applications | Biossay, Fluorometric |
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| Other Names | n/a |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | 12601 |
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| Order / More Info | Beta-Galactosidase Assay Kit (green fluorescence) from AAT BIOQUEST, INC. |
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| Product Specific References | n/a |
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