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Product Name | Collagen, Recombinant, Human |
Description | Purified by proprietary chromatographic techniques. ≥95% as determined by: 1. RP-HPLC. 2. Anion-exchange FPLC3. Reducing and non-reducing SDS-PAGE Silver Stained gel. Recombinant Human Collagen produced in E. coli is a non-glycosylated polypeptide chain a molecular mass of 96kD containing human collagen's cDNA transcribed reversely from mRNA. Amino Acid Sequence: The sequence of the first 15 N-terminal amino acids was determined and was found to be MDPVVLQRRDWENPG. Amino Acid %: Asp 0.39; Thr 0.16; Ser 5.70; Pro 18.87; Gly 29.17; Ala 13.38; Val 0.71; Met 0.18; Ile 0.16; Leu 8.04; Tyr 0.90; Phe 0.15; Lys 0.27; His 0.15; Arg 0.03; Gln 17.40; Hydro Pro 4.34. Biological Activity: 1. Cultivation of BHK-21 and 2BS cell line BHK-21 or 2BS cells were plated onto the bottom of the 96-well cell culture plates at the density of 1x10e3cells/well, respectively, with 0.1ml RPMI1640 contained 10% fetal bovine serum in the well. BHK-21 or 2BS cells were incubated in standard conditions (at 37°C wit |
Size | 5ug, 20ug |
Concentration | n/a |
Applications | n/a |
Other Names | n/a |
Gene, Accession, CAS # | n/a |
Catalog # | C7510-02 |
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Order / More Info | Collagen, Recombinant, Human from UNITED STATES BIOLOGICAL |
Product Specific References | n/a |
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