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Product Name | Ischemia Modified Albumin Assay Kit |
Description | Ischemia Modified Albumin Assay Kit is an Assay Kit by Albumin Cobalt Binding Test.This reagent is used to determine the IMA content in human serum.Normal albumin exists as active form in serum or plasma, N - terminal of albumin combine with Co+ while adding cobalt chloride solution, then concentration of free Co+ in the solution decrease. The serum or plasma of patients with myocardial ischemia contains more IMA, the ability of albumin binding to Co+ decreased while adding the same amount of cobalt chloride solution, so free Co+ concentration higher. The higher concentration of free Co+ formed more colored product s when adding dithiothreito l (DTT). Colorimetric test at a specific wavelength, the absorbance is proportional to the concentration of free Co+. The IMA concentration in the sample can be calculated by comparing the calibrator result with same processing. table, th, td { border: 1px solid black; border-collapse: collapse; } th, td { padding: 10px; } R1: Detection Reagent 1 R2: Detection Reagent 2 Tris Buffer → 20 mmol/l Good's Buffer → 50 mmol/l Cobalt Dichloride → 45 mg/l Dithiothreitol (DTT) → 1.5 mmol/l Preservatives → Appropriate Stabilizer → Appropriate Preservatives → Appropriate This assay kit is suitable for automatic or semi - automatic biochemical analyzer with 505nm and 700nm wavelength.TSerum samples. Samples testing should be completed within 3 hours after collected. Otherwise, the samples should be cryopreserved and avoid repeated freeze - thaw cycles. IMA in the samples remain stable for 3 months at - 20°C.. Range: 40 U/ml - 150 U/ml. |
Size | n/a |
Concentration | n/a |
Applications | RUO |
Other Names | Ischemia-modified Albumin; IMA |
Gene, Accession, CAS # | n/a |
Catalog # | abx098440 |
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Order / More Info | Ischemia Modified Albumin Assay Kit from ABBEXA |
Product Specific References | n/a |
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