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| Product Name | Malate Dehydrogenase (MDH) Assay Kit (Colorimetric) |
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| Description | Malate dehydrogenase (MDH) (EC 1.1.1.37) is an enzyme which reversibly catalyzes the oxidation of L-malate to oxaloacetate in the presence of NAD. There are 2 isoforms in eukaryotic cells: MDH1 and MDH2. MDH1 found in the cytoplasm and plays a key part in the malate-aspartate shuttle for transporting malate into the mitochondria. MDH2 is a mitochondrial enzyme which participates in the TCA cycle that reversibly converts L-malate into oxaloacetate. Higher MDH activities are found in some neurodegenerative diseases such as Alzheimer's disease. This non-radioactive, colorimetric MDH assay is based on the reduction of the tetrazolium salt MTT in a NADH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at 565 nm. The increase in absorbance at 565 nm is proportional to the enzyme activity. Sample types: culture media, erythorocytes, plasma, serum, tissue |
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| Size | n/a |
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| Concentration | n/a |
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| Applications | For quantitative determination of L-malate dehydrogenase enzyme activity and drug effects on malate dehydrogenase metabolism |
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| Other Names | n/a |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | LS-K273 |
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| Order / More Info | Malate Dehydrogenase (MDH) Assay Kit (Colorimetric) from LIFESPAN BIOSCIENCES INC. |
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| Product Specific References | n/a |
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