产品名称 AKT1, internal Antibody from ROCKLAND IMMUNOCHEMICALS INC.
产品货号 K-I51
产品价格 现货询价,电话:010-67529703
产品规格 1 Pair
产品品牌 ROCKLAND IMMUNOCHEMICALS INC.
产品概述
产品详情
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Antigenic SpecificityAKT1, internal
Clone14E5.A2.B2.H9
Host SpeciesMouse
Reactive Specieshuman
IsotypeIgG2a kappa
FormatProtein A purified
Size1 Pair
Concentration1.0 mg/ml
ApplicationsELISA, Flow Cytometry, Immunohistochemistry, Western Blot. Anti-AKT1 Antibody with the matched secondary are suitable for ELISA, immunohistochemistry, and western blotting. Expect a band approximately 56 kDa in size corresponding to AKT1 protein by western blotting in the appropriate cell lysate or extract. This primary monoclonal antibody reacts with human AKT. Specific conditions for reactivity should be optimized by the end user. For immunohistochemistry we recommend the use of fresh frozen tissues. Attempts at staining paraffin-embedded formalin fixed tissues were negative. No pre-treatment of sample is required.
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DescriptionThis primary and secondary antibody pair has been carefully chosen to give best resutls. Anti-AKT1 antibody is directed against human AKT1. The antibody detects both unphosphorylated and phosphorylated forms of the protein. Anti-AKT1 antibody was purified from ascites by Protein A chromatography. Cross reactivity with AKT1 from other species has not been determined, however, the sequence of the immunogen shows 85% identity to mouse and 92% identity with rat, therefore, cross reactivity is expected.HRP secondary antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. This product shows balanced reactivity to Mouse IgG1, IgG2a, IgG2b and IgG3 proteins and is suitable to screen IgG class hybridoma clones. Minimal cross reactivity is observed against other Mouse immunoglobulin classes or light chain proteins. This antibody pair is sufficient for up to 5 mini blots at 7.5 x 8 cm2 (1,800 cm2).
ImmunogenAnti-AKT1 Antibody was produced by repeated immunizations with a synthetic peptide corresponding to internal residues of human AKT1 protein. Anti-Mouse IgG HRP secondary antibody was produced by repeated immunizations with Mouse IgG whole molecule
Other NamesAKT 1 antibody pair, AKT-1, PKB antibody, PKB gamma antibody, PKBGAMMA antibody, PRKBG antibody, Protein kinase Akt 1 antibody, Protein kinase B gamma antibody, RAC-gamma serine/threonine-protein kinase, RAC-PK-gamma, primary and secondary antibody pair, Primary+Secondary pair, matched antibody pair
Gene, Accession #AKT1, Gene ID: 207, NCBI: NP_001014431.1, UniProt: P31749
Catalog #K-I51
Price
Order / More InfoAKT1, internal Antibody from ROCKLAND IMMUNOCHEMICALS INC.
Product Specific Referencesn/a
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