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| Product Name | Gliadin Ab IgA ELISA Kit |
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| Description | The enzyme-linked immunosorbent assay (ELISA) is intended for the quantitative or qualitative determination of IgA antibodies in human serum, directed against gliadin. This product is for Research Use Only; International customers contact your representative to receive information about the CE marked version. The immobilized antigen is a gliadin preparation purified from wheat gluten. The test is fast (incubation time 30 / 30 / 30 minutes) and flexible (divisible solid phase, ready-to-use reagents). Six calibrators allow quantitative measurements; a negative and a positive control check the assay performance. The wells of the solid phase are coated with gliadin. On this surface, the following immunological reactions take place: 1st reaction: gliadin-specific antibodies present in the sample bind to the immobilized antigen, forming the antigen-antibody complex. Then, non-bound sample components are washed away from the solid phase. 2nd reaction: A second antibody, directed at human IgA antibodies and conjugated with horse-radish peroxidase (HRP), is added. This conjugate binds to the complex. Then, excess conjugate is washed away from the solid phase. 3rd reaction: The enzyme-labelled complex converts a colorless substrate into a blue product. The degree of color development reflects the concentration of gliadin antibodies (IgA) in the sample. |
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| Size | 96 wells |
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| Concentration | n/a |
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| Applications | RUO |
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| Other Names | EIA, enzyme immunoassay, assay |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | EIA4069R |
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| Order / More Info | Gliadin Ab IgA ELISA Kit from DRG INTERNATIONAL INC. |
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| Product Specific References | n/a |
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