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| Product Name | 1,25-Dihydroxy Vitamin D EIA |
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| Description | The IDS 1,25-Dihydroxy Vitamin D EIA kit is a complete assay system for the purification of 1,25D in patient samples by immunoextraction followed by quantitation by EIA. Patient samples are delipidated and 1,25D extracted from potential cross-reactants by incubation for 90 minutes with a highly specific solid phase monoclonal anti-1,25D. The immunoextraction gel is then washed and purified 1,25D eluted directly into glass assay tubes. Reconstituted eluates and calibrators are incubated overnight with a highly specific sheep anti-1,25D. Then a portion of this is incubated for 90 minutes with shaking in microplate wells which are coated with a specific anti-sheep antibody. 1,25D linked to biotin is then added and the plate shaken for a further 60 minutes before aspiration and washing. Enzyme (horseradish peroxidase) labelled avidin is added and binds selectively to complexed biotin and, following a further wash step, colour is developed using a chromogenic substrate (TMB). The absorbance of the stopped reaction mixtures are read in a microtitre plate reader, colour intensity developed being inversely proportional to the concentration of 1,25D. |
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| Size | 96 wells |
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| Concentration | n/a |
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| Applications | colorimetric ELISA or EIA |
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| Other Names | 1,25-dihydroxyvitamin D; 1,25D; 1,25OH D kit, assay, immunoassay |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | AC-62F1 |
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| Order / More Info | 1,25-Dihydroxy Vitamin D EIA from IMMUNODIAGNOSTIC SYSTEMS |
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| Product Specific References | n/a |
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