| 产品详情 |
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| Antigenic Specificity | Rock-2 phospho Y256 |
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| Clone | polyclonal |
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| Host Species | Rabbit |
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| Reactive Species | human |
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| Isotype | n/a |
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| Format | immunoaffinity purified |
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| Size | 100 µg |
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| Concentration | 1.0 mg/mL |
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| Applications | ELISA, Immunohistochemistry, Western Blot. This affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 164 kDa in size corresponding to phosphorylated ROCK-2 by western blotting in the appropriate cell lysate or extract. This phospho-specific polyclonal antibody reacts with human ROCK-2 pY256 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide. |
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| Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
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| Description | This product was affinity purified from monospecific antiserum by immunoaffinity chromatography using phospho-peptide coupled to agarose beads followed by solid phase adsorption against nonphospho-peptide. This antibody is specific for human ROCK-2 protein phosphorylated at Y256. A BLAST analysis was used to suggest cross-reactivity with ROCK-2 from human, mouse, rat, chicken, dog, bovine, Xenopus and Drosophila based on a 100% homology with the immunizing sequence. Cross-reactivity with ROCK-2 from other sources has not been determined. |
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| Immunogen | This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding Y256 of human ROCK-2. |
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| Other Names | rabbit anti-ROCK2 antibody, Rho-associated protein kinase 2, Rho-associated coiled-coil-containing protein kinase 2, p164 ROCK-2, Rho kinase 2, Rho-associated coiled-coil-containing protein kinase II, ROCK-II, ROCK-2, ROCK 2 |
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| Gene, Accession # | ROCK2, Gene ID: 9475, NCBI: 4520225, UniProt: O75116 |
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| Catalog # | 600-401-998 |
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| Price | |
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| Order / More Info | Rock-2 phospho Y256 Antibody from ROCKLAND IMMUNOCHEMICALS INC. |
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| Product Specific References | Akhtar S, Yousif MH, Dhaunsi GS, Sarkhouh F, Chandrasekhar B, Attur S, Benter IF. (2013) Activation of ErbB2 and Downstream Signalling via Rho Kinases and ERK1/2 Contributes to Diabetes-Induced Vascular Dysfunction. PLoS One. 2013 Jun 27;8(6):e67813. doi: 10.1371/journal.pone.0067813. Print 2013. |
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