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| Antigenic Specificity | CHK2 phospho T68 |
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| Clone | polyclonal |
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| Host Species | Rabbit |
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| Reactive Species | human |
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| Isotype | n/a |
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| Format | immunoaffinity purified |
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| Size | 100 µg |
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| Concentration | 1.0mg/ml |
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| Applications | ELISA, Immunoprecipitation, Western Blot. This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 61 kDa in size corresponding to CHK2 by western blotting in the appropriate cell lysate or extract. Less than 1% reactivity is observed against the non-phosphorylated form of the immunizing peptide. This antibody is phospho specific for pT68 of CHK2. |
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| Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
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| Description | This affinity purified antibody is directed against the phosphorylated form of human CHK2 at the pT68 residue. The product was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross adsorbed against the non-phosphorylated form of the immunizing peptide. Reactivity occurs against human CHK2 pT68 protein and the antibody is specific for the phosphorylated form of the protein. Reactivity with non-phosphorylated human CHK2 is minimal by ELISA. The antibody does not cross-react with Chk2 phosphorylated at other sites. A BLAST analysis was used to suggest reactivity with this protein from human and chimpanzee based on 100% homology for the immunogen sequence. Cross reactivity with CHK2 protein from mouse and rat may occur as sequence homology varies by one amino acid residues in this sequence (90% homology). Cross reactivity with CHK2 homologues from other sources has not been determined. |
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| Immunogen | This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding aa 64-73 of Human CHK2. |
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| Other Names | rabbit anti-CHK2 pT68 Antibody, Serine/threonine-protein kinase Chk2, CHK-2, CHK 2, CDS1, RAD53, CHEK2, Checkpoint kinase 2, Hucds1 |
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| Gene, Accession # | CHEK2, Gene ID: 11200, NCBI: NP_001005735.1, UniProt: O96017 |
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| Catalog # | 600-401-280 |
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| Price | |
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| Order / More Info | CHK2 phospho T68 Antibody from ROCKLAND IMMUNOCHEMICALS INC. |
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| Product Specific References | Lu YP, Lou YR, Peng QY, Xie JG, Nghiem P, Conney AH. (2008) Effect of caffeine on the ATR/Chk1 pathway in the epidermis of UVB-irradiated mice. Cancer Res. 2008 Apr 1;68(7):2523-9. doi: 10.1158/0008-5472.CAN-07-5955. |
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