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| Antigenic Specificity | SARS 3Cl Protease |
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| Clone | polyclonal |
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| Host Species | Rabbit |
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| Reactive Species | sars |
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| Isotype | n/a |
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| Format | Protein A purified |
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| Size | 500 µg |
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| Concentration | 5.0 mg/mL |
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| Applications | ELISA, IF Microscopy, Western Blot. This protein A purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 34 kDa in size corresponding to SARS 3CL Protease by western blotting in the appropriate cell lysate or extract. |
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| Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
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| Description | This protein A purified antibody is directed against SARS Coronavirus 3CL Protease. The product was purified from monospecific antiserum by protein A affinity purification. BLAST analysis was used to suggest reactivity with related Coronavirus proteins. Cross reactivity with homologues from other sources has not been determined. |
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| Immunogen | This protein A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a purified recombinant protein corresponding to full length SARS Coronavirus 3CL Protease. Lifesensors Inc. (www.lifesensors.com) prepared the 3CL Protease as follows: SUMO-3CL protease fusion was expressed in E. coli in LB medium and purified by Ni-NTA resin affinity chromatography (Qiagen). After the fusion was cleaved by the SUMO Protease (LifeSensors), the SUMO tag and protease were subtracted from the 3CL protease using MAC and the 3CL protease was finally purified using Anion Exchange Chromatography with the Macro-Prep High Q resin (BioRad) and size exclusion chromatography. |
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| Other Names | rabbit anti-Sars CoV 3CL Protease Antibody, 3CL PRO antibody, 3CLp antibody, nsp5 antibody |
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| Gene, Accession # | 3CL-PRO, Gene ID: 1489680, NCBI: 29837498, UniProt: P0C6U8 |
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| Catalog # | 200-401-A51 |
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| Price | |
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| Order / More Info | SARS 3Cl Protease Antibody from ROCKLAND IMMUNOCHEMICALS INC. |
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| Product Specific References | n/a |
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