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Antigenic Specificity | Collagen Type II |
Clone | polyclonal |
Host Species | Rabbit |
Reactive Species | human, mouse, rat, bovine, sheep |
Isotype | n/a |
Format | immunoaffinity purified |
Size | 100 µg |
Concentration | 1.0 mg/mL |
Applications | ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot. Anti-Collagen antibodies have been used for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, immunoprecipitation, immunohistochemistry, native (non-denaturing, non-dissociating) PAGE, and western blotting for highly sensitive qualitative analysis. |
Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
Description | Collagen II Antibody has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities. Typically negligible cross reactivity against other types of collagens was detected by ELISA against purified standards. Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type II collagens and has negligible cross-reactivity with Type I, III, IV, V or VI collagens. Non-specific cross reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible. |
Immunogen | Collagen Type II from human knee cartilage and bovine nasal cartilage. |
Other Names | rabbit anti-Collagen Type II antibody, Collagen alpha-1 (II) chain, Alpha-1 type II collagen, Cartilage collagen antibody, Chondrocalcin antibody, COL2A1 antibody |
Gene, Accession # | COL2A1, Gene ID: 1280, NCBI: P02458.3, UniProt: P02458 |
Catalog # | 600-401-104-0.1 |
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Order / More Info | Collagen Type II Antibody from ROCKLAND IMMUNOCHEMICALS INC. |
Product Specific References | Shunya Kondo, Chisa Shukunami, Yoko Morioka, Naoya Matsumoto, Rei Takahashi, Junseo Oh, Tadao Atsumi, Akihiro Umezawa, Akira Kudo, Hitoshi Kitayama, Yuji Hiraki, Makoto Noda. (2007) Dual effects of the membrane-anchored MMP regulator RECK on chondrogenic differentiation of ATDC5 cells. Journal of Cell Science 120, 849-857. doi:10.1242/jcs.03388. |
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