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Antigenic Specificity | Collagen I alpha 1 propeptide, internal |
Clone | polyclonal |
Host Species | Rabbit |
Reactive Species | human, mouse |
Isotype | n/a |
Format | immunoaffinity purified, no preservative |
Size | 100 µL |
Concentration | Titrated value sufficient to run approximately 10 mini blots. |
Applications | ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot. Anti-Collagen I, alpha 1 propeptide Antibody is suitable for use in ELISA, Western Blotting and IHC. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 180 kDa in size corresponding to collagen I alpha I polypeptide in human lung fibroblast extracts or other appropriate tissues or lysates. Collagen I alpha 1 propeptide antibody also works well for IHC on paraformaldehyde-fixed sections with a simple antigen-retrieval protocol (incubate slides for 20 minutes at 90° C in 10 mM sodium citrate (pH 6.0)/ 0.1 % Tween-20). Note that in paraffin sections of formaldehyde-fixed fibrotic mouse lung tissue, the antibody recognizes collagen I molecules that are still associated with the cells in which they were synthesized. |
Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
Description | Anti-Collagen I alpha 1 propeptide antibody is directed against collagen I alpha 1 propeptide (C-terminal region). Anti-Collagen I alpha 1 propeptide antibody was affinity purified from monospecific antiserum by immunoaffinity purification. Anti-Collagen I alpha 1 propeptide antibody recognizes Collagen I alpha 1 propeptide from human and mouse sources. Cross reactivity with Collagen I alpha 1 propeptide from other species has not been determined. However, reactivity is also expected from most vertebrate species based on very high sequence homology. |
Immunogen | Anti-collagen I, alpha 1 propeptide Antibody was produced in rabbit by repeated immunizations with a synthetic peptide corresponding to amino acid residues specific to collagen 1, alpha 1 propeptide conjugated to KLH. |
Other Names | Alpha-1 type I collagen, COLA1A1 |
Gene, Accession # | COL1A1, Gene ID: 1277, UniProt: P02452 |
Catalog # | 600-401-D19 |
Price | |
Order / More Info | Collagen I alpha 1 propeptide, internal Antibody from ROCKLAND IMMUNOCHEMICALS INC. |
Product Specific References | n/a |