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| Antigenic Specificity | C-MYB PT486, N-terminus |
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| Clone | polyclonal |
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| Host Species | Rabbit |
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| Reactive Species | mouse |
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| Isotype | n/a |
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| Format | affinity purified |
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| Size | 100 µg |
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| Concentration | 1.51 |
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| Applications | ELISA, Western Blot. This affinity purified antibody has been tested for use in ELISA and western blotting using M1 cells which express endogenous wild-type c-Myb protein and in COS7 cells transfected with c-Myb. Specific conditions for reactivity and detection of phosphorylated c-Myb should be optimized by the end user. Expect a band approximately ~75 kDa in size corresponding to phosphorylated c-Myb by western blotting in the appropriate cell lysate or extract. |
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| Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
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| Description | This affinity-purified antibody is directed against the phosphorylated form of mouse c-Myb protein at the pT486 residue. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross adsorbed against the non-phosphorylated form of the immunizing peptide. Reactivity occurs against mouse c-Myb pT486 protein and the antibody is specific for the phosphorylated form of the protein. This antibody reacts with endogenous c-Myb protein and also SUMOylated overexpressed c-Myb protein in transfected Cos7 cells. Reactivity with non-phosphorylated mouse c-Myb is minimal by ELISA and western blot. A BLAST analysis was used to suggest reactivity with c-Myb from mouse based on a 100% homology with the immunizing sequence. Expect reactivity with c-Myb from rat, pig, human, horse, platypus, opossum, orangutan, spider monkey, bonobo, gorilla, and chimpanzee; with a v-myb myeloblastosis viral oncogene homolog, all isoforms (also avian version) from human; and with a protooncogene c-myb, and a v-myb myeloblastosis viral oncogene homolog (avian), both from cattle, all based on a 93% homology with the immunizing sequence. Cross-reactivity with c-Myb from other sources has not been determined. |
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| Immunogen | This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of mouse c-Myb protein surrounding amino acid residue 486. |
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| Other Names | rabbit anti-c-Myb pT486 Antibody, Proto-oncogene c-Myb antibody, C myb antibody, MYB antibody, Transcriptional activator Myb, cmyb antibody |
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| Gene, Accession # | MYB, Gene ID: 17863, NCBI: 110556654, UniProt: P06876 |
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| Catalog # | 600-401-C47 |
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| Price | |
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| Order / More Info | C-MYB PT486, N-terminus Antibody from ROCKLAND IMMUNOCHEMICALS INC. |
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| Product Specific References | Bies, J., Sramko, M., & Wolff, L. (2013). Stress-induced Phosphorylation of Thr486 in c-Myb by p38 Mitogen-activated Protein Kinases Attenuates Conjugation of SUMO-2/3. Journal of Biological Chemistry, 288(52), 36983-36993. |
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