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| Antigenic Specificity | Telomerase catalytic subunit, C-terminus |
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| Clone | polyclonal |
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| Host Species | Rabbit |
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| Reactive Species | human |
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| Isotype | n/a |
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| Format | serum |
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| Size | 100 µg |
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| Concentration | 1.0 mg/mL |
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| Applications | ELISA, Immunohistochemistry, IF Microscopy, Immunoprecipitation, Western Blot. This affinity purified antibody has been tested for use in immunoblotting, immunoprecipitation, and immunofluorescence microscopy. In these assays, the antibody detects ectopically-expressed hTERT and high levels of endogenous hTERT. Although it has been reported that this antibody reacts with mouse TERT (mTERT) (see Drissi, et al. 2001), the binding to mTERT is considerably weaker and less specific than the binding to hTERT (not shown). A SY5Y cell nuclear extract can be used as a positive control. To detect TERT, fix cells in 2% paraformaldehyde (in PBS) for 10'. Wash the slides twice in PBS for 5' each. Permeabilize the cells in 0.5% NP-40 for 10'. Wash as before in PBS. Block the cells using PBG buffer (0.2% cold water fish gelatin (Sigma G-7765) and 0.5% BSA in PBS) for 20' at room temperature. Incubate in primary antibody (diluted in PBG) for 1-2 hours at RT or overnight at 4°C. Wash the slides three times in PBG for 5' each. Incubate with secondary antibody (diluted in PBG) for 1 hour at RT in the dark. Wash the slides three times in PBG for 5' each. Mount in DAPI-containing medium.In immunoblot assays, whole cell or nuclear extracts were loaded at a concentration of 100 µg protein per well. A working dilution of 1:500 anti-TERT antibody was used followed by a 1:3,000 dilution of horseradish peroxidase- conjugated goat anti-rabbit IgG as the secondary antibody. For immunofluorescence microscopy staining, a working dilution of 1:500 was used followed by a 1:200 dilution of rhodamine-conjugated donkey anti-rabbit IgG as a secondary antibody. Immunoprecipitation was performed using 20 µL of protein A beads and 2 µL of the anti-TERT serum per 1mg protein from cell lysate. A working dilution of 1:500 is also suggested for immunohistochemistry. |
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| Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
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| Description | This antiserum primarily detects hTERT, but several non-specific bands appear on immunoblots. In immunofluorescence microscopy assays, staining with anti-TERT-16 was specific to the nuclei of cells with ectopic TERT expression. |
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| Immunogen | This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the carboxy terminal end of hTERT (accession number AF018167). |
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| Other Names | rabbit anti-TERT antibody, rabbit anti-Telomerase catalytic subunit antibody, hTERT, Telomerase reverse transcriptase, HEST2, Telomerase-associated protein 2, TP2, EST2, TCS1, TRT |
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| Gene, Accession # | TERT, Gene ID: 7015, NCBI: O14746.1, UniProt: O14746 |
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| Catalog # | 600-401-252 |
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| Price | |
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| Order / More Info | Telomerase catalytic subunit, C-terminus Antibody from ROCKLAND IMMUNOCHEMICALS INC. |
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| Product Specific References | Wu, Y.L., et al. (2006) Immunodetection of human telomerase reverse-transcriptase (hTERT) re-appraised: nucleolin and telomerase cross paths. J.Cell Sci. 119: 2797-2806.Ahmed S, JF Passos, MJ Birket, T Beckmann, S Brings, H Peters, MA Birch-Machin, T von Zglinicki, G. Saretzki. Telomerase does not counteract telomere shortening but protects mitochondrial function under oxidative stress. J Cell Sci. 2008 Apr 1;121(Pt 7):1046-53. Epub 2008 Mar 11.Rubis, B., Holysz, H., Gladych, M., Toton, E., Paszel, A., Lisiak, N., ... & Rybczynska, M. (2013). Telomerase downregulation induces proapoptotic genes expression and initializes breast cancer cells apoptosis followed by DNA fragmentation in a cell type dependent manner. Molecular biology reports, 40(8), 4995-5004.Radan L, Hughes CS, Teichroeb JH, Vieira Zamora FM, Jewer M, Postovit LM, Betts DH. (2014) Microenvironmental regulation of telomerase isoforms in human embryonic stem cells. Stem Cells Dev. 2014 Sep 1;23(17):2046-66. doi: 10.1089/scd.2013.0373. Epub 2014 Jun 17.Gizard F, Heywood EB, Findeisen HM, Zhao Y, Jones KL, Cudejko C, Post GR, Staels B, Bruemmer D. (2010) Telomerase activation in atherosclerosis and induction of telomerase reverse transcriptase expression by inflammatory stimuli in macrophages. Arterioscler Thromb Vasc Biol. 2011 Feb;31(2):245-52. doi: 10.1161/ATVBAHA.110.219808. Epub 2010 Nov 24. |
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