| 产品详情 |
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| Antigenic Specificity | Goat Ig |
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| Clone | polyclonal |
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| Host Species | Rabbit |
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| Reactive Species | goat |
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| Isotype | n/a |
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| Format | agarose conjugate |
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| Size | 2.5 mL |
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| Concentration | 2.0 |
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| Applications | Immunoprecipitation, Western Blot. Upon initial use of this product, we recommend that the vial be inverted several times to get the beads into suspension. We recommend to use a large bore pipet to pipet up the liquid for use.For storage of the opened vial, we recommend that the vial cap be sealed with parafilm to help prevent evaporation of the buffer.Procedure: Preparation of Immunoprecipitated Sample for SDS-PAGE:1. Preclear cell lysate: Add 50 µl of anti-goat IgG beads and 500 µl of cell lysate sample to an eppendorf tube and incubate on ice for 30 minutes. Spin at 10,000xg for 3 minutes and transfer the supernatant to a new eppendorf tube.2. Immunoprecipitation: Add 5 µg of primary antibody to the eppendorf tube containing the precleared lysate. Incubate on ice for 1 hour. Add 50 µl of Anti-Goat IgG Beads. Incubate for 1 hour on a rocking platform. Spin the eppendorf tube at 10000xg for 1 minute. Remove supernatant completely and wash the (pelleted) beads 3 times with 500 µl of Lysis Buffer.3. Prepare sample for SDS-PAGE: After the last wash, aspirate supernatant, and add 100 µl Laemmli Buffer (with 50 mM DTT or 2% β-mercaptoethanol, final) or NuPAGE LDS Sample Buffer (Invitrogen Cat#NP0007) containing NuPAGE Sample Reducing Agent (dithiothreitol; Invitrogen Cat#NP0004) to bead pellet. Vortex and heat to 90-100°C for 10 minutes. Spin at 10000xg for 3 minutes, collect supernatant, and load onto the gel. Avoid loading anti-goat Ig beads.Note: The supernatant can be stored at -20°C for future use. After thawing, add fresh NuPAGE Sample Reducing Agent (dithiothreitol) and heat as above. Centrifuge the sample at 10000xg for 1 minute in a microcentrifuge to pellet any anti-goat Ig beads and immediately transfer an aliquot of the supernatant to gel wells. |
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| Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
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| Description | TrueBlot® anti-Goat Ig IP Agarose Beads are a suspension of activated agarose beads coupled with rabbit anti-goat IgG. It is suitable for precipitation of goat IgGs used as the primary antibodies in immunoprecipitation assays. The beads are in suspension and will settle upon storage. Prior to use, mix the vial gently (do not vortex) to ensure delivery of proper bead volume. |
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| Immunogen | n/a |
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| Other Names | Anti-Goat immunoglobulin Gamma, Agarose-conjugated IgG, Rb-a-Gt IgG, Rabbit-anti-Goat IgG, TrueBlot, TrueBlot for immunoprecipitation, IP Agarose beads for TrueBlot. |
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| Gene, Accession # | n/a |
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| Catalog # | 00-8844-25 |
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| Price | |
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| Order / More Info | Goat Ig Antibody from ROCKLAND IMMUNOCHEMICALS INC. |
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| Product Specific References | n/a |
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