| 产品详情 |
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| Antigenic Specificity | IL-6 |
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| Clone | polyclonal |
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| Host Species | Rabbit |
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| Reactive Species | human |
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| Isotype | n/a |
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| Format | serum, no preservative |
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| Size | 25 µL |
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| Concentration | 80 mg/mL |
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| Applications | ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot. This antiserum against IL-6 has been tested for use in neutralizations, ELISA, immuno-histochemistry, radioimmunoassay, immunoprecipitation and immunoblotting. Reactivity in other immunoassays is unknown. For immunoblotting use the supernatants or lysates of 2 x 106 endotoxin-stimulated human peripheral blood mononuclear cells (PBMC). PBMC are stimulated for 24 hours with 1% (v/v) human serum plus 10 ng/mL E.coli LPS. For immunohistochemistry either paraffin fix or cryofix tissue. For immunoprecipitation, pre-clearing with a non-specific rabbit IgG is helpful to reduce background. |
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| Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
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| Description | Anti-IL-6 antiserum detects recombinant and native IL-6 present in body fluids and cell supernatants in various assays (ie. IL-1 stimulated IL-6 production from fibroblasts). In Western blot analysis of natural cell products or human body fluids, multiple bands of IL-6 will appear due to the variable amount of glycosylation on the molecule. The antiserum is also useful for neutralization of human of IL-6 activity in bioassays. For neutralization, incubate the sample with a 1:400 dilution of the antiserum for at least 4 hours before being tested. A control of similarly diluted normal rabbit IgG (heat inactivated) is recommended. In neutralization experiments in vitro, this antibody does not result in enhanced activity of IL-6. However, because antibodies to IL-6 may act as a soluble receptor in vivo, some antibodies to IL-6 act as carriers and enhance IL-6 activity. This product has minimal reactivity with mouse IL-6. |
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| Immunogen | This whole rabbit serum was prepared by repeated immunizations with recombinant human IL-6 produced in E.coli. |
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| Other Names | HSF, Hybridoma growth factor, Hybridoma plasmacytoma growth factor, IFNB2, IL 6, IL6 protein, Interferon beta 2, BSF2, CDF, rabbit anti-Interleukin 6 Antibody, rabbit anti-IL-6 |
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| Gene, Accession # | IL6, Gene ID: 3569, NCBI: NP_000591.1, UniProt: P05231 |
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| Catalog # | 109-401-310S |
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| Price | |
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| Order / More Info | IL-6 Antibody from ROCKLAND IMMUNOCHEMICALS INC. |
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| Product Specific References | n/a |
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