| 产品详情 |
| Edit |   |
| Antigenic Specificity | Rad9, p Ser1260 |
| Clone | polyclonal |
| Host Species | Rabbit |
| Reactive Species | yeast |
| Isotype | n/a |
| Format | immunogen affinity purified |
| Size | 0.1 mg |
| Concentration | 0.41 mg/ml |
| Applications | Western Blot, ELISA. This phospho specific polyclonal antibody was tested by immunoblotting and ELISA. Data from both immunoblotting and ELISA indicate the antibody is reactive with the phosphorylated form of the immunizing peptide and minimally reactive with the non-phosphorylated form of the immunizing peptide. Immunoblotting detects yeast Rad9 protein. No reactivity is expected against the human or mouse analogs of RAD9. Reactivity against RAD9 from other sources is unknown. Cross reactivity may occur with auto-phosphorylated Rad53 kinase. Although not tested, this antibody is likely functional by IHC and IP.This product has been assayed against 0.1 ug of phosphorylated peptide in a standard capture ELISA using TMB TMBE-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:5,000 is suggested for this product. Minimal reactivity was detected against the non-phosphorylated form of the immunizing peptide. This antibody appears - be specific for the active form of the protein. Dilute the antibody 1:100 - 1:500 for immunoblotting. Researchers should determine optimal titers for other applications. |
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| Description | The Rad9 [p Ser1260] Antibody from Novus Biologicals is a rabbit polyclonal antibody to Rad9. This antibody reacts with yeast. The Rad9 [p Ser1260] Antibody has been validated for the following applications: Western Blot, ELISA. This affinity purified antibody is directed against the phosphorylated form of yeast Rad9 at the pS1260 residue |
| Immunogen | This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to phosphorylated form of aa 1249-1263 of 1309 of yeast Rad9 protein conjugated to KLH. |
| Other Names | cell cycle checkpoint control protein RAD9A, DNA repair exonuclease rad9 homolog A, EC 3.1.11.2, hRAD9, RAD9, RAD9 (S. pombe) homolog, RAD9 homolog A (S. pombe) |
| Gene, Accession # | RAD9A, Gene ID: 5883, Accession: NP_004575, SwissProt: NP_004575 |
| Catalog # | NBP1-77981 |
| Price | |
| Order / More Info | Rad9, p Ser1260 Antibody from NOVUS BIOLOGICALS, LLC |
| Product Specific References | n/a |
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