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| Product Name | FAM-Spacer-Phe-CMK Green FLISP Assay Kit |
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| Description | Description: Detect changes in intracellular chymotrypsin-like serine protease enzyme activity in whole living cells with the FLISP, Fluorescent-Labeled Inhibitors of Serine Proteases, product line of assay kits. This assay utilizes a reagent (FSFCK) containing a phenylalanine (F) chymotrypsin-targeting amino acid residue linked on the amino terminus with a carboxyfluorescein (FAM) fluorescent reporter tag. The carboxyl end of the F amino acid residue contains a reactive chloromethyl ketone (CMK |
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| Size | 25 tests, 100 tests, |
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| Concentration | n/a |
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| Applications | n/a |
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| Other Names | n/a |
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| Gene, Accession, CAS # | FAM-Spacer-Phe-CMK |
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| Catalog # | MBS258007 |
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| Order / More Info | FAM-Spacer-Phe-CMK Green FLISP Assay Kit from MYBIOSOURCE INC. |
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| Product Specific References | Czapinska, H. and J. Otlewski. 1999. Structural and energetic determinants of the S1-site specificity in serine proteases. Eur. J. Biochem. 260: 571-595. Grabarek, J., M. Dragan, B.W. Lee, G.L. Johnson, and Z. Darzynkiewicz. 2002. Activation of chymotrypsin-like serine proteases during apoptosis detected by affinity-labeling of the enzymatic center with fluoresceinated inhibitor. Int. J. Oncol. 20: 225-233. Zhu, H., D. Dinsdale, E.S. Alnemri, and G.M. Cohen. 1997. Apoptosis in human monocytic THP-1 cells involves several distinct targets of N-tosyl-L-phenylalanyl chloromethyl ketone (TPCK). Cell Death Diff. 4: 590-599. Grabarek, J., M. Dragan, B.W. Lee, G.L. Johnson, and Z. Darzynkiewicz. 2002. Activation of chymotrypsin-like serine protease(s) during apoptosis detected by affinity-labeling of the enzymatic center with fluoresceinated inhibitor. Int. J. Oncol. 20: 225-233. Grabarek, J., L. Du, G.L. Johnson, B.W. Lee, D. J. Phelps, and Z. Darzynkiewicz. 2002. Sequential activation of caspases and serine proteases (Serpases). Cell Cycle 1: 124-131. O'Connell, A.R., B.W. Lee, and C. S. Cox. 2006. Caspase-dependent activation of chymotrypsin-like proteases mediates nuclear events during Jurkat T cell apoptosis. Biochem. Biophys. Res. Comm. 345: 608-616. O'Connell, AR., C. Holohan, A. Torriglia, B.W. Lee, and C.S. Cox. 2006. Characterization of a serine protease-mediated cell death program activated in human leukemia cells. Exp. Cell Res. 312: 27-39. |
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