| 产品详情 |
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| Product Name | FAM-Leu-DAP Green FLISP Assay Kit |
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| Description | Description: Detect changes in intracellular chymotrypsin-like serine protease enzyme activity in whole living cells with the FLISP, Fluorescent-Labeled Inhibitors of Serine Proteases, product line of assay kits. This assay utilizes a leucine (L) chymotrypsin-targeting amino acid residue linked on the amino terminus with either a carboxyfluorescein (FAM) fluorescent reporter tag. The carboxyl end of the F or L amino acid residue contains a reactive group that targets the catalytic site of serine |
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| Size | 25 tests, 100 tests, |
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| Concentration | n/a |
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| Applications | n/a |
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| Other Names | n/a |
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| Gene, Accession, CAS # | FAM-Leu-DAP |
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| Catalog # | MBS258022 |
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| Order / More Info | FAM-Leu-DAP Green FLISP Assay Kit from MYBIOSOURCE INC. |
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| Product Specific References | Czapinska, H. and J. Otlewski. 1999. Structural and energetic determinants of the S1-site specificity in serine proteases. Eur. J. Biochem. 260: 571-595. Grabarek, J., M. Dragan, B.W. Lee, G.L. Johnson, and Z. Darzynkiewicz. 2002. Activation of chymotrypsin-like serine proteases during apoptosis detected by affinity-labeling of the enzymatic center with fluoresceinated inhibitor. Int. J. Oncol. 20: 225-233. Zhu, H., D. Dinsdale, E.S. Alnemri, and G.M. Cohen. 1997. Apoptosis in human monocytic THP-1 cells involves several distinct targets of N-tosyl-L-phenylalanyl chloromethyl ketone (TPCK). Cell Death Diff. 4: 590-599. Grabarek, J., L. Du, G.L. Johnson, B.W. Lee, D. J. Phelps, and Z. Darzynkiewicz. 2002. Sequential activation of caspases and serine proteases (Serpases). Cell Cycle 1: 124-131. Oleksyszyn, J., and J. Powers. 1991. Irreversible inhibition of serine proteases by peptide derivatives of (?-aminoalkyl)phosphonate diphenyl esters. Biochemistry 30: 485-493. Abuelyaman, A.S., D.S. Jackson, D. Hudig, S.L. Woodard, and J.C. Powers. 1997. Synthesis and kinetic studies of diphenyl 1-(N-peptidylamino) alkanephosphonate esters and their biotinylated derivatives as inhibitors of serine proteases and probes for lymphocyte granzymes. Arch. Biochem. Biophys. 344: 271-280. Powers, J.C., J.L. Asgian, O.D. Ekici, and K.E. James. 2002. Irreversible inhibitors of serine, cysteine, and threonine proteases. Chem. Rev. 102: 4639-4750. |
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