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| Product Name | QuickDetect™ 5-lipoxygenase (5-LOX) (Human) ELISA Kit |
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| Description | BioVision's 5-lipoxygenase ELISA kit is based on Sandwich ELISA method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to 5-LOX. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for 5-LOX is added to each Microelisa stripplate well and after incubation, all unbound components are washed away. The TMB substrate solution is added to each well. The wells that contain 5-LOX and HRP conjugated 5-LOX antibody will appear blue in color and turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of 5-LOX. The concentration of 5-LOX in the samples can be calculated by comparing the OD of the samples to the standard curve. |
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| Size | 96 assays |
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| Concentration | n/a |
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| Applications | Detection range: 1U/L - 480 U/L, Assay Precision: Intra-Assay: CV<10%, Inter-Assay: CV<12%, CV (%) = SD/mean X 100, Sensitivity: 1U/L |
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| Other Names | ALOX5, 5-LOX, Arachidonate 5-lipoxygenase |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | E4704-100 |
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| Price | |
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| Order / More Info | QuickDetect™ 5-lipoxygenase (5-LOX) (Human) ELISA Kit from BIOVISION, INC. |
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| Product Specific References | n/a |
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