| 产品详情 |
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| Antigenic Specificity | APE |
| Clone | 13B8E5C2 |
| Host Species | Mouse |
| Reactive Species | human, mouse, rat, primate |
| Isotype | IgG2b |
| Format | Protein G purified |
| Size | 0.1 mg |
| Concentration | 1.0 mg/ml |
| Applications | Western Blot, Simple Western, Chromatin Immunoprecipitation, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Gel Super Shift Assays, Proximity Ligation Assay. Gel Super Shift Assays was reported in scientific literature. In Western blot, this antibody detects a single band at 37 kDa. In IHC, it can be competitively inhibited from recognizing the APE1 antigen in tissues using APE1 protein. It can also be used on frozen and fixed-paraffin sections and cytospin preps. In IHC-P, staining was observed in the nucleus of a human breast cancer xenograft. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. Use in Proximity Ligation Assay reported in scientific literature (PMID: 27808278). |
| Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
| Description | The APE Antibody (13B8E5C2) from Novus Biologicals is a mouse monoclonal antibody to APE. This antibody reacts with human, mouse, rat, primate. The APE Antibody (13B8E5C2) has been validated for the following applications: Western Blot, Simple Western, Chromatin Immunoprecipitation, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Gel Super Shift Assays, Proximity Ligation Assay. |
| Immunogen | Purified human APE1 [Uniprot: P27695] |
| Other Names | AP endonuclease 1, APE, APE1, APE-1, APEAPEX nuclease (multifunctional DNA repair enzyme), APENAP endonuclease class I, APEX deoxyribonuclease (apurinic or apyrimidinic), APEX nuclease, APEX nuclease (multifunctional DNA repair enzyme) 1, Apurinic-apyrimidinic endonuclease 1, APXAP lyase, DNA-(apurinic or apyrimidinic site) lyase, EC 3.1, EC 4.2.99.18, HAP1apurinic/apyrimidinic (abasic) endonuclease, multifunctional DNA repair enzyme, protein REF-1, redox factor 1, Redox factor-1, REF-1, REF1 apurinic/apyrimidinic exonuclease |
| Gene, Accession # | APEX1, Gene ID: 328, Accession: P27695, SwissProt: P27695 |
| Catalog # | NB100-116 |
| Price | |
| Order / More Info | APE Antibody from NOVUS BIOLOGICALS, LLC |
| Product Specific References | PubMed: 10690545, 10906132, 11205241, 11280790, 11309329, 11562758, 11809696, 11846302, 11850801, 12566303, 12767347, 14973123, 15084314, 15210853, 15218023, 15531919, 15746182, 16243814, 16373707, 16481390, 17195915, 17332344, 17522063, 17565005, 17566060, 17766324, 18784354, 18809583, 19268524, 19414382, 19864471, 20032196, 20096707, 20332233, 20363599, 20525204, 21207372, 21261287, 21325978, 21769563, 21991345, 22024594, 22027539, 22441348, 22488727, 22580151, 22583700, 22709585, 22710064, 22713669, 22841870, 23145165, 23154201, 23199242, 23268706, 23418439, 23874636, 24127576, 24337968, 24400589, 24459704, 24914806, 25423885, 25728837, 25762636, 25790189, 26608791, 26876348, 26981776, 27019630, 27177180, 27230458, 27593437, 27608656, 27637330, 27655688, 27794043, 27808278, 27994014, 28751279, 28986522, 30705092, 30745413 |
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