| 产品详情 |
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| Antigenic Specificity | Alkaline Phosphatase, Bacterial |
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| Clone | L2 |
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| Host Species | Mouse |
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| Reactive Species | n/a |
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| Isotype | IgG2a |
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| Format | unpurified, no preservative |
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| Size | 0.1 ml |
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| Concentration | n/a |
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| Applications | Western Blot, Immunocytochemistry/Immunofluorescence, Immunoprecipitation. Western Blot at 42-45kDa on SDS-PAGE,reducing gels for natural E. coli alkaline phosphatase monomer. Alkaline phosphatase fusion proteins will vary depending upon the target fusion protein. Immunocytochemistry/Immunofluorescence : reacts with E.coli. AP fusion protein targets in acetone fixed cell preparations. 1:4000, other fixatives or conditions untested. ASSAY: Preparation of E. coli TnphoA transformants: E. coli strain CC118 was transformed with plasmid pGEM-3Z containing TnphoA insertional mutations in the p101 gene of Mycoplasma hyorhinis, which encodes a protein with a typical N-terminal prokaryotic single peptide (Yogev et al. 1991). Identification of fusion protein with MAB1012 transformants: Transformants are grown in 2XYT medium to OD600=0.6. Cells were centrifuged 3 minutes at 10,000 x g, suspended in SDS-PAGE sample buffer, heated at 100C for 5 minutes, frozen and thawed and centrifuged as above at room temperature to remove insoluble material. The sample is applied at 9% to a SDS-PAGE gel, and Western immunoBlot is performed as described (Yogev et al. 1991). Immunoprecipitation: 5ul of antibody per 500ul of lysate in RIPA or 0.5% triton X-100 solutions. |
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| Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
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| Description | The Alkaline Phosphatase, Bacterial Antibody (L2) from Novus Biologicals is a mouse monoclonal antibody to Alkaline Phosphatase, Bacterial. This antibody reacts with virus. The Alkaline Phosphatase, Bacterial Antibody (L2) has been validated for the following applications: Western Blot, Immunocytochemistry/Immunofluorescence, Immunoprecipitation. Specificity: Alkaline phosphatase (AP) |
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| Immunogen | F201C1B. |
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| Other Names | BAP, ECK0378, JW0374, Pho A, PhoA |
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| Gene, Accession # | phoA, Gene ID: 249, Accession: P00634, SwissProt: P00634 |
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| Catalog # | NBP2-29868 |
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| Price | |
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| Order / More Info | Alkaline Phosphatase, Bacterial Antibody from NOVUS BIOLOGICALS, LLC |
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| Product Specific References | n/a |
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