| 产品详情 |
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| Antigenic Specificity | Aggrecan |
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| Clone | BC-3 |
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| Host Species | Mouse |
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| Reactive Species | human, mouse, rat, porcine, bovine, canine, equine, feline, guinea pig, rabbit, sheep |
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| Isotype | IgG1 kappa |
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| Format | supernatant |
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| Size | 1 ml |
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| Concentration | n/a |
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| Applications | Western Blot, ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen. This Aggrecan antibody is useful in Immunohistochemistry, ELISA and Western blot. With IHC the antibody works in formalin- or paraformaldehyde-fixed paraffin embedded sections as well as either alcohol-fixed frozen sections or un-fixed snap-frozen sections. In Western blot the antibody detects a variety of epitopes between 50 and 250 kDa. Samples must be deglycosylated using 0.01 Units Chondroitinase ABC (Sigma), 0.01 Units Keratanase (Seikagaku) and 0.0001 Units Keratanase II (Seikagaku) per 10ug S-GAG of non-deglycosylated aggrecan for optimal epitope recognition. See Little et al. and Caterson et al. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 27185069). |
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| Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
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| Description | The Aggrecan Antibody (BC-3) from Novus Biologicals is a mouse monoclonal antibody to Aggrecan. This antibody reacts with human, mouse, rat, porcine, bovine, canine, equine, feline, guinea pig, rabbit, sheep. The Aggrecan Antibody (BC-3) has been validated for the following applications: Western Blot, ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen. Recognizes the aggrecanase (ADAMTS-1, -4 & -5)-generated N-terminal neoepitope ARG after cleavage between amino acids EGE and ARG within the interglobular domain of aggrecanase-catabolised aggrecan |
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| Immunogen | ARGSV... synthetic peptide conjugate. |
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| Other Names | AGC1SEDK, aggrecan, aggrecan core protein, Cartilage-specific proteoglycan core protein, Chondroitin sulfate proteoglycan 1, Chondroitin sulfate proteoglycan core protein 1, CSPCP, CSPG1aggrecan 1, CSPGCP, large aggregating proteoglycan, MSK16AGCAN |
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| Gene, Accession # | ACAN, Gene ID: 176, Accession: P16112, SwissProt: P16112 |
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| Catalog # | NB600-504 |
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| Price | |
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| Order / More Info | Aggrecan Antibody from NOVUS BIOLOGICALS, LLC |
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| Product Specific References | PubMed: 12009333, 20633676, 22394620, 23325424, 27185069, 30858866 |
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