| 产品详情 |
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| Antigenic Specificity | Fas Ligand/TNFSF6 |
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| Clone | A11 |
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| Host Species | Rat |
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| Reactive Species | mouse |
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| Isotype | IgM |
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| Format | Protein G purified |
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| Size | 0.1 mg |
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| Concentration | 1.0 mg/ml |
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| Applications | Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen. Immunohistochemical localization of FasL in adult mouse testis using the A11 antibody. Method: Cryostat sections (6-8um) were air dried, fixed in 4% paraformaldehyde in PBS and used immediately. Sections were incubated for 1 hr at room temperature (RT) with 20 ug/ml of A11 antibody in PBS, 0.2% BSA, 0.05% Tween 20. After rinsing in PBS, a FITC-conjugated anti-rat IgM was added for 30 min., slides washed again in PBS and visualized using a fluorescence microscope. Paraffin sections: Dewax in xylene (2x10 min), EtOH 100% (2x10 min), block endogeneous peroxidase (0.6% H2O2 in Methanol 10 min), rehydrate through graded EtOH (100%, 95%, 70%), rinse in H20, unmask epitopes by heating slides in citrate buffer (0.01M, pH 6.0) 2x5 min in microwave oven (650-750W), cool down to RT 20 min, wash 1x in PBS, incubate with A11 20 ug/ml antibody, wash in PBS, incubate in anti-rat IgM HRP (ALX-211-055), wash in PBS, develop in appropriate substrate solution (DAB or AEC). |
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| Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
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| Description | The Fas Ligand/TNFSF6 Antibody (A11) from Novus Biologicals is a rat monoclonal antibody to Fas Ligand/TNFSF6. This antibody reacts with mouse. The Fas Ligand/TNFSF6 Antibody (A11) has been validated for the following applications: Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen. |
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| Immunogen | Synthetic peptide corresponding to aa 196-220 of mouse FasL (CD95L; APO-1L; CD178). |
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| Other Names | apoptosis (APO-1) antigen ligand 1, Apoptosis antigen ligand, APT1LG1CD95L, APTL, CD178, CD178 antigen, CD95-L, Fas antigen ligand, Fas ligand, Fas ligand (TNF superfamily, member 6), FASLCD95 ligand, TNFSF6FasL, tumor necrosis factor (ligand) superfamily, member 6, tumor necrosis factor ligand superfamily member 6 |
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| Gene, Accession # | FASLG, Gene ID: 356 |
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| Catalog # | NBP1-97519 |
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| Price | |
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| Order / More Info | Fas Ligand/TNFSF6 Antibody from NOVUS BIOLOGICALS, LLC |
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| Product Specific References | PubMed: 11359858, 12473659, 7495745, 7510950, 8609166, 8627156, 8910274, 9077524, 9620682, 9780162 |
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