| 产品详情 |
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| Antigenic Specificity | Cytokeratin 18 |
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| Clone | C-04 |
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| Host Species | Mouse |
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| Reactive Species | human, mouse, mammal |
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| Isotype | IgG1 |
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| Format | Protein A purified |
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| Size | 0.1 mg |
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| Concentration | 1 mg/ml |
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| Applications | Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin, CyTOF-ready. Predicted molecular weight: 45 kDa. Western Blot - Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing SDS-PAGE sample buffer. Application note: Non-reducing conditions. SDS-PAGE (10% separating gel) ELISA - The antibody C-04 has been tested as the capture antibody in a sandwich ELISA for analysis of Cytokeratin 18 in combination with antibody DA-7 and with the antibody DC-10. This antibody is CyTOF ready. |
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| Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
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| Description | The Cytokeratin 18 Antibody (C-04) from Novus Biologicals is a mouse monoclonal antibody to Cytokeratin 18. This antibody reacts with human, mouse, mammal. The Cytokeratin 18 Antibody (C-04) has been validated for the following applications: Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin, CyTOF-ready. The antibody C-04 reacts with Cytokeratin 18 (45 kDa), a member of intermediate filaments subfamily represented in epithelial tissues |
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| Immunogen | Cytoskeleton preparation of epidermal carcinoma cell line A431. |
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| Other Names | Cell proliferation-inducing gene 46 protein, cell proliferation-inducing protein 46, CK-18, CYK18, cytokeratin 18, cytokeratin-18, K18, keratin 18, keratin, type I cytoskeletal 18, keratin-18 |
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| Gene, Accession # | KRT18, Gene ID: 3875 |
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| Catalog # | NB500-306 |
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| Price | |
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| Order / More Info | Cytokeratin 18 Antibody from NOVUS BIOLOGICALS, LLC |
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| Product Specific References | PubMed: 22020958 |
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