| 产品详情 |
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| Antigenic Specificity | KLRAQ1 |
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| Clone | V/10 SM-M10 |
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| Host Species | Mouse |
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| Reactive Species | human, porcine, bovine, rabbit |
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| Isotype | IgG1 |
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| Format | Protein A/G purified |
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| Size | 0.1 mg |
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| Concentration | 1 mg/ml |
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| Applications | Western Blot, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin. Western Blot (see application notes below). Suggested blocking buffer is TBS-Tween with 2% BSA. Suggested dilution buffer is TBS-Tween with 0.05% sodium azide. Preferred gel percentage is 5% (see application notes). Immunohistochemistry on frozen and paraffin embedded tissue sections. Suggested fixation for frozen tissue sections is acetone fix for 6 minutes at room temperature. For formalin fixed paraffin embedded tissue sections: microwave in 0.01M citrate buffer (pH 6.0) for 8-10 minutes (note that all microwaves differ and adjustments may need to be made) follow with enzyme digestion (0.01% pronase for 10 mintues). Suggested blocking agent is fetal bovine serum. The antibody has also been used successfully on methyl-Carnoy fixed tissue. Immunocytochemistry/Immunofluorescence Immunoprecipitation. Suggested extraction buffer is 20 mM Tris-HCl, pH 7.4, 150 mM NaCl, 1% Triton X-100, 0.1% SDS, 0.5% deoxycholic acid-NaCl and 0.5 mM PMSF. Final reaction volume is 1 mL and suggested capture agent is agarose conjugated anti-mouse IgG. APPLICATION NOTES FOR MAB3572 WESTERN Blot To achieve good resolution of myosin heavy chain isoforms with distinct molecular weight (200 - 2004 kDa), the following procedure should be followed: 1). Pyrophoshate extraction buffer for sample preparation (see below); run SDS-PAGE in 5% gel. Important: for better resolution of the MHC bands, use electrophoretic buffer with pH 8.2 (i.e. 0.1 less than standard), and prepare resolving gel (5%) with pH 9.0 (not 8.8 as usual). Also help thorough degasing of the resolving gel mixture (H2O, acrylamide, EDTA, pH 9.0, before (!) adding SDS, TEMED and APS). Run SDS-PAGE longer than after the dye front runs off (use 200 kDa MW markers and let it's 200 kDa band run at least to the middle of 8X8 gel (using a big size gel (not the mini-gel!) will enhance the quality of MHC band resolution). Pyrophosphate extraction buffer: (40mM Na4P2O7x10H2O, 1mM MgCl2, 1mM EGTA (add KOH to dissolve EGTA), PMSF, pH 9.5). To extract acto-myosin from tissues/cells, shake minced tissue or cells in cold extraction buffer 1 hr on ice bath (0oC), centrifuge @10,000g for 10 min at +2-8 oC, take supernatant and mix it 1:1 with standard Laemmli sample buffer, boil, run SDS-PAGE in 5% gel (see above). |
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| Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
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| Description | The KLRAQ1 Antibody (V/10 SM-M10) from Novus Biologicals is a mouse monoclonal antibody to KLRAQ1. This antibody reacts with human, porcine, bovine, rabbit. The KLRAQ1 Antibody (V/10 SM-M10) has been validated for the following applications: Western Blot, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin. Specificity: Smooth muscle myosin, heavy chain |
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| Immunogen | Crude smooth muscle extract from normal human adult uterus. |
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| Other Names | CCDC128, coiled-coil domain containing 128, Coiled-coil domain-containing protein 128, FLJ16566, KLRAQ motif containing 1, KLRAQ motif-containing protein 1, MGC111781 |
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| Gene, Accession # | PPP1R21, Gene ID: 129285, Accession: Q6ZMI0, SwissProt: Q6ZMI0 |
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| Catalog # | NBP2-29850 |
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| Price | |
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| Order / More Info | KLRAQ1 Antibody from NOVUS BIOLOGICALS, LLC |
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| Product Specific References | n/a |
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