| 产品详情 |
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| Antigenic Specificity | Complement C3 |
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| Clone | 11H9 |
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| Host Species | Rat |
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| Reactive Species | mouse |
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| Isotype | IgG2a |
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| Format | Alexa Fluor 647 conjugate |
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| Size | 0.1 ml |
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| Concentration | n/a |
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| Applications | Western Blot, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen. For IHC-F: Tissue sections were fixed in acetone. Antigen retrieval was performed using 50% formic acid for 5 min or microwaved (700 W) in antigen unmasking solution. For IHC-P: free-floating brain tissue pretreated with 50% formic acid for 5 min. or microwaved (700W) in antigen unmasking solution for 2-5min. Blocking endogenous persoxidase by treatment with 3% H2O2 in pBS 20 min at 25 degrees C. Blocking 2hrs, 2% BSA in PBS. For IF: Fixation in 4% paraformaldehyde in PBS pH 7.4. Vibratome sections of 4 um. Pretreated with 3% hydrogen peroxide for 20 min to quench endogenous peroxidases. Microwaved in antigen unmasking solution for 2-5 minutes as antigen retrieval. |
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| Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
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| Description | The Complement C3 Antibody (11H9) [Alexa Fluor® 647] from Novus Biologicals is a rat monoclonal antibody to Complement C3. This antibody reacts with mouse. The Complement C3 Antibody (11H9) [Alexa Fluor® 647] has been validated for the following applications: Western Blot, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen. Specificity: Mouse Complement C3 and its activation products, C3b, iC3b, C3d and C3dg |
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| Immunogen | C57BL/6 thymocytes saturated with rat anti-Thy-1 monoclonal antibody of IgG2b subclass (RmT1) |
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| Other Names | AHUS5, ARMD9, ASP, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1, complement C3, complement component 3, complement component C3, CPAMD1acylation-stimulating protein cleavage product |
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| Gene, Accession # | C3, Gene ID: 718 |
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| Catalog # | NB200-540AF647 |
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| Price | |
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| Order / More Info | Complement C3 Antibody from NOVUS BIOLOGICALS, LLC |
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| Product Specific References | n/a |
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