| 产品详情 |
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| Antigenic Specificity | Vimentin |
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| Clone | VI-RE/1 |
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| Host Species | Mouse |
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| Reactive Species | human |
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| Isotype | IgG1 |
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| Format | Protein A purified |
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| Size | 0.1 mg |
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| Concentration | 1.0 mg/ml |
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| Applications | Western Blot, Flow Cytometry, ELISA, Immunocytochemistry/Immunofluorescence, CyTOF-ready. Western Blot - Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with reducing Laemmli SDS-PAGE sample buffer. Boil for 3 min in water bath. Application note: Reducing conditions. SDS-PAGE (10% separating gel). ELISA - The antibody VI-RE/1 recognizes different epitope on human vimentin than the antibody VI-01 (IgM). This antibody is CyTOF ready. |
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| Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
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| Description | The Vimentin Antibody (VI-RE/1) from Novus Biologicals is a mouse monoclonal antibody to Vimentin. This antibody reacts with human, mouse (negative), porcine (negative). The Vimentin Antibody (VI-RE/1) has been validated for the following applications: Western Blot, Flow Cytometry, ELISA, Immunocytochemistry/Immunofluorescence, CyTOF-ready. The antibody VI-RE/1 reacts with human vimentin, a 57 kDa intermediate filament protein expressed on a wide variety of mesenchymal and mesodermal cell types |
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| Immunogen | Bacterially expressed full-length human vimentin |
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| Other Names | FLJ36605, vimentin |
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| Gene, Accession # | VIM, Gene ID: 7431 |
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| Catalog # | NB500-512 |
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| Price | |
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| Order / More Info | Vimentin Antibody from NOVUS BIOLOGICALS, LLC |
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| Product Specific References | n/a |
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