| 产品详情 |
| Edit |   |
|---|
| Antigenic Specificity | HMGN1/HMG14, p Ser20, p Ser24 |
|---|
| Clone | polyclonal |
|---|
| Host Species | Rabbit |
|---|
| Reactive Species | human, mouse, rat, porcine, bovine, canine, chicken, orangutan, sheep, Xenopus |
|---|
| Isotype | n/a |
|---|
| Format | immunogen affinity purified |
|---|
| Size | 0.1 mg |
|---|
| Concentration | 1.2 mg/ml |
|---|
| Applications | Western Blot, ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin. Expect a band approximately 17 kDa in size corresponding - phosphorylated HMGN proteins by western blotting in the appropriate cell lysate or extract. Less than 0.5% reactivity is observed against the non-phosphorylated form of the immunizing peptide. This antibody is phospho specific for pS20 and pS24 of HMGN proteins. The antibody was tested against HMGN1 by performing a standard phopshorylation in vitro assay using HMGN1 as a substrate and Msk1 as a kinase . This kinase specifically phosphorylates S20 and S24 of HMGN1 . The reaction was resolved on SDS PAGE and immunoblotted, using antibody diluted at 1:1,000 and 1:5,000. The antibody shows strong positive signal for Msk-phosphorylated HMGN1 and no cross-reaction for non-treated HMGN1. |
|---|
| Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
|---|
| Description | The HMGN1/HMG14 [p Ser20, p Ser24] Antibody from Novus Biologicals is a rabbit polyclonal antibody to HMGN1/HMG14. This antibody reacts with human, mouse, rat, porcine, bovine, canine, chicken, orangutan, sheep, xenopus. The HMGN1/HMG14 [p Ser20, p Ser24] Antibody has been validated for the following applications: Western Blot, ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin. This antibody is directed against phosphorylated human HMGN protein at pS20 and pS24 residues (other HMGN proteins have this conserved sequence but at other residue positions) |
|---|
| Immunogen | This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 19-28 of human HMGN1 protein (see below). |
|---|
| Other Names | FLJ31471, High mobility group nucleosome-binding domain-containing protein 1, high-mobility group (nonhistone chromosomal) protein 14, high-mobility group nucleosome binding 1, high-mobility group nucleosome binding domain 1, HMG14FLJ27265, MGC104230, MGC117425, non-histone chromosomal protein HMG-14, nonhistone chromosomal protein HMG-14 |
|---|
| Gene, Accession # | HMGN1, Gene ID: 3150, Accession: P05114, SwissProt: P05114 |
|---|
| Catalog # | NBP1-78102 |
|---|
| Price | |
|---|
| Order / More Info | HMGN1/HMG14, p Ser20, p Ser24 Antibody from NOVUS BIOLOGICALS, LLC |
|---|
| Product Specific References | PubMed: 26156854 |
|---|
| 产品资料 |
|
|