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| Product Name | JC-10 Mitochondrion Membrane Potential Assay Kit (for microplate assays) |
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| Description | Cell Meter™ JC-10 Mitochondrion Membrane Potential Assay Kit *Optimized for Microplate Assays*. Although JC-1 is widely used in many labs, its poor water solubility makes it hard to use for some applications. Even at 1 µM concentration, JC-1 tends to precipitate in aqueous buffer. JC-10 has been developed to be a superior alternative to JC-1 where high dye concentration is desired. Compared to JC-1, our JC-10 has much better water solubility. JC-10 is capable of entering selectively into mitochondria, and changes reversibly its color from green to orange as membrane potentials increase. This property is due to the reversible formation of JC-10 aggregates upon membrane polarization that causes shifts in emitted light from 520 nm (i.e., emission of JC-10 monomeric form) to 570 nm (i.e., emission of J-aggregate). When excited at 490 nm, the color of JC-10 changes reversibly from green to greenish orange as the mitochondrial membrane becomes more polarized. This Cell Meter™ JC-10 Mitochondrial Membrane Potential Assay Kit enable you to monitor mitochondrial membrane potential changes using a simple microplate reader while all the other commercial JC-1 assay kits require the use of a flow cytometer. Our kit provides the most robust method to monitor mitochondrial membrane potential changes, and can be readily used for screening a large compound library. |
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| Size | 500 tests |
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| Concentration | n/a |
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| Applications | Biossay, Fluorometric |
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| Other Names | n/a |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | 22800 |
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| Order / More Info | JC-10 Mitochondrion Membrane Potential Assay Kit (for microplate assays) from AAT BIOQUEST, INC. |
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| Product Specific References | n/a |
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