| 产品详情 |
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| Antigenic Specificity | HIF-1 alpha |
| Clone | H1alpha67 |
| Host Species | Mouse |
| Reactive Species | human, mouse, rat, porcine, bovine, canine, ferret, monkey, primate, rabbit, sheep, Xenopus |
| Isotype | IgG2b |
| Format | Protein G purified |
| Size | 0.1 ml |
| Concentration | 1.0 mg/ml |
| Applications | Western Blot, Chromatin Immunoprecipitation, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Gel Super Shift Assays, Proximity Ligation Assay, Tissue Culture Substratum, Ligand Activation, Knockout Validated. ChIP usage was reported in scientific literature. ELISA usage was reported in scientific literature (PMID: 20042684). Gel Super Shift Assays usage was reported in scientific literature (PMID: 22411794). Ligand Activation usage was reported in scientific literature (PMID: 26147748). In WB, a band can be seen at 120 kDa representing HIF-1 alpha in induced tissues and cells. Multiple bands may be seen at 100-120 kDa representing post-translational modification of HIF-1 alpha. For WB, testing on nuclear extracts is recommended. Knock Out Validation was reported in scientific literature (PMID: 26861754). Use in proximity ligation assay reported in scientific literature (PMID 27595394). |
| Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
| Description | The HIF-1 alpha Antibody (H1alpha67) from Novus Biologicals is a mouse monoclonal antibody to HIF-1 alpha. This antibody reacts with human, mouse, rat, porcine, bovine, canine, ferret, monkey, primate, rabbit, sheep, xenopus. The HIF-1 alpha Antibody (H1alpha67) has been validated for the following applications: Western Blot, Chromatin Immunoprecipitation, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Gel Super Shift Assays, Proximity Ligation Assay, Tissue Culture Substratum, Ligand Activation, Knockout Validated. |
| Immunogen | A fusion protein containing amino acids 432 - 528 of human HIF-1 alpha [UniProt# Q16665]. |
| Other Names | anti-HIF-1 alpha, anti-HIF1A, ARNT-interacting protein, Basic-helix-loop-helix-PAS protein MOP1, BHLHE78, Class E basic helix-loop-helix protein 78, H1alpha67, HIF-1 alpha, HIF1A, HIF-1-alpha, HIF1-alpha, hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor), hypoxia-inducible factor 1-alpha, Member of PAS protein 1, member of PAS superfamily 1, MOP1HIF1-ALPHA, PAS domain-containing protein 8, PASD8alpha subunit (basic helix-loop-helix transcription factor) |
| Gene, Accession # | HIF1A, Gene ID: 3091, SwissProt: Q16665 |
| Catalog # | NB100-105 |
| Price | |
| Order / More Info | HIF-1 alpha Antibody from NOVUS BIOLOGICALS, LLC |
| Product Specific References | PubMed: 10582706, 10702253, 10758161, 10773083, 10973499, 10987269, 11292861, 11292862, 11410504, 11443623, 11562758, 11641398, 11676860, 11698256, 11840443, 11859074, 11860493, 11884613, 12004076, 12060624, 12193413, 12226811, 12351678, 12538497, 12750296, 14645546, 1466820, 14668208, 15024076, 15026807, 15048086, 15063691, 15063696, 15132953, 15155569, 15180977, 15185341, 15364715, 15374969, 15557337, 15696961, 15771621, 15774498, 15833858, 15857276, 15935515, 15985433, 15987772, 16024619, 16050983, 16099863, 16161047, 16166593, 16204079, 16204254, 16214846, 16284088, 16424008, 16424110, 16432225, 16477043, 16481368, 16504308, 16505488, 16507742, 16507764, 16509823, 16527906, 16540671, 16543236, 16555123, 16585189, 16603692, 16611993, 16627790, 16627934, 16645044, 16677866, 16740971, 16760477, 16762988, 16772336, 16790524, 16815840, 16873559, 16877351, 16878133, 16885151, 16899558, 16912095, 16928739, 16946160, 16951228, 16971531, 16973934, 16982742, 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