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| Product Name | Mouse TrueBlot® Western Blot Kit |
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| Description | The Mouse TrueBlot® Western Blot Kit contains the critical supporting reagents, buffers, and substrates for immunoprecipitation and Western blotting of samples using TrueBlot second step immunoblotting reagents in conjunction with your own primary IP antibody and primary (Mouse IgG) Western blotting antibody.TrueBlot technology enables unhindered detection of protein bands of interest which would otherwise be obscured by the presence of reduced and denatured heavy and light chain immunoglobulin in the blot (as detected by the conventional immunoblotting HRP anti-mouse IgG reagent).Mouse IgG TrueBlot® ULTRA is the unique horseradish peroxidase conjugated anti-mouse IgG immunoblotting second step reagent which enables detection of immunoblotted target protein bands, without hindrance by interfering immunoglobulin heavy and light chains from your IP antibody. Use it in place of your usual HRP anti-mouse IgG immunoblotting second step reagent. It is easy to generate publication-quality IP/WB data with Mouse IgG TrueBlot® ULTRA.Mouse IgG TrueBlot ULTRA is ideal for use in protocols involving immunoblotting of immunoprecipitated proteins. TrueBlot preferentially detects the non-reduced form of mouse IgG over the reduced, SDS-denatured form of IgG. When the immunoprecipitate is fully reduced immediately prior to SDS-gel electrophoresis, reactivity of Mouse IgG TrueBlot ULTRA with the 55 kDa heavy chains and the 23 kDa light chains of the immunoprecipitating antibody is minimized thereby eliminating interference by the heavy and light chains of the immunoprecipitating antibody in IP/immunoblotting applications. Applications include studies examining post-translational modification (e.g., phosphorylation or acetylation) or protein-protein interactions. |
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| Size | 1 kit |
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| Concentration | n/a |
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| Applications | Immunoprecipitation, Western Blot. Mouse IgG TrueBlot® ULTRA is provided as 1000X solution. In order to conserve reagent, we recommend incubating the blots from minigels in sealed bags (removing as much air as possible) with minimal volume (2-3 mls). If used conservatively at 2.5mls/blot, cat. 18-8817-31 (50µL) will yield enough reagent for 20 blots.Mouse IgG TrueBlot® ULTRA. An HRP-conjugated second step reagent reacting with mouse IgGs for optimal signal detection in immunoprecipitation/immunoblotting experiments.Note that there are three key procedural considerations:1. Protein A or G beads may be used with the mouse, goat and sheep TrueBlot secondaries but not with the rabbit TrueBlot secondary. Use of protein A or G beads with the rabbit TrueBlot will result in contaminating bands.2. Immunoprecipitate should be completely reduced.3. Milk should be used as the blocking protein for the immunoblot. A sample of Anti-alpha-Tubulin (MOUSE) Monoclonal Antibody Cat #(200-301-880S), has been provided to be used as a loading control at the user's discretion. Dilutions are to be optimized by the user.The Mouse TrueBlot® Western Blot Kit components are sufficient for 20-25 miniblots.Components:1. Mouse IgG TrueBlot ULTRA: 50 µl. 18-8817-31 2. TrueBlot Enhancer Solution: 25 ml3. TrueBlot Blocker: 10 g4. TrueBlot Assay Buffer: 30 ml. 20X5. TrueBlot Substrate A: 12.5 ml6. TrueBlot Substrate B: 12.5 ml7. Western Blot Incubation Tray8. Anti-alpha-Tubulin (MOUSE) Monoclonal Antibody - 200-301-880S |
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| Other Names | HRP, TrueBlot, HRP TrueBlot ULTRA, Peroxidase TrueBlot, TrueBlot for IP/WB, TrueBlot for immunoprecipitation, TrueBlot for western blotting |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | 88-8887-31 |
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| Order / More Info | Mouse TrueBlot® Western Blot Kit from ROCKLAND IMMUNOCHEMICALS INC. |
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| Product Specific References | n/a |
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