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| Antigenic Specificity | DYKDDDDK (FLAG® tag) |
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| Clone | 29E4.G7 |
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| Host Species | Mouse |
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| Reactive Species | n/a |
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| Isotype | IgG2a kappa |
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| Format | agarose conjugate |
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| Size | 1 mL |
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| Concentration | 1.0 mg |
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| Applications | Immunoprecipitation. Anti-DYKDDDDK Affinity Gel is optimally suited for immunoprecipitation and purification of FLAG® tagged fusion proteins. Anti-DYKDDDDK Affinity Gel antibody recognizes the FLAG® epitope tag fused to either the amino- or carboxy- terminal ends or an internal location of targeted fusion proteins. The epitope tag peptide sequence was first derived from the 11-amino-acid leader peptide of the gene-10 product from bacteriophage T7. DYKDDDDK is the most commonly used hydrophilic octapeptide tag. Use D-Y-K-D-D-D-D-K peptide (p/n 000-000-383) for competitive elution to recover fusion protein (see protocol). Anti-FLAG® is a registered trademark of Sigma-Aldrich.Refer to the Protocol for complete instructions for use including preferred buffers for elution. Do not use buffers that may denature the anti-DYKDDDDK antibody. |
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| Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
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| Description | Anti-DYKDDDDK Affinity Gel is a purified mouse IgG2a monoclonal antibody coupled to activated agarose. This product is intended for purification of proteins containing the FLAG® epitope tag sequence. Binding Specificity: Anti-DYKDDDDK Affinity Gel binds the FLAG® epitope tag sequence (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) fused to the amino terminal, carboxy terminal or internal locations of targeted recombinant proteins expressed in transfected or transformed cells. D-Y-K-D-D-D-D-K peptide (p/n 000-000-383) is recommended for competitive elution to recover fusion protein (see protocol). |
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| Immunogen | Anti-DYKDDDDK Affinity Gel antibody was produced in mice by repeated immunizations with a synthetic peptide corresponding to the FLAG® epitope tag peptide DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) conjugated to KLH. |
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| Other Names | mouse anti-FLAG® tag agarose gel, mouse anti-DYKDDDDK agarose gel, Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys, D-Y-K-D-D-D-D-K, DDK, FLAG, FLAG antibody, anti-Flag, anti-DDK, DDK antibody |
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| Gene, Accession # | n/a |
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| Catalog # | 200-350-383 |
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| Price | |
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| Order / More Info | DYKDDDDK (FLAG® tag) Antibody from ROCKLAND IMMUNOCHEMICALS INC. |
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| Product Specific References | n/a |
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