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Antigenic Specificity | c-erbB2, extracellular |
Clone | SP3 |
Host Species | Rabbit |
Reactive Species | human |
Isotype | IgG |
Format | supernatant |
Size | 100 µL |
Concentration | n/a |
Applications | ELISA, Immunohistochemistry, Western Blot. erbB2 Antibody monoclonal antibody contains TBS/1% BSA and is suitable for ELISA, immunohistochemistry and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 185 kDa in size corresponding to c-erbB2 protein by western blotting in the appropriate cell lysate or extract. For immunohistochemistry, samples should be formalin fixed and paraffin embedded. Deparaffinize slides using xylene or xylene alternatives and graded alcohols. Staining requires boiling of sections in 10 mM citrate buffer pH 6.0 for 10 min followed by cooling at RT for 20 min. C-erbB2 is found in human breast carcinoma tissue at the cell membrane. |
Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
Description | Anti-erbB2 Antibody is clarified tissue culture supernate. The antibody is specific for human c-erbB2 protein. Cross-reactivity with c-erbB2 protein from other sources has not been determined. |
Immunogen | erbB2 monoclonal antibody was produced by repeated immunizations with a recombinant protein corresponding to amino acid residues encoding the extracellular domain of human c-erbB2 protein. The hybridoma was produced by fusing New Zealand White rabbit splenocytes and myeloma cells using conventional technology. |
Other Names | rabbit anti-c-erbB2 Antibody, Receptor tyrosine-protein kinase erbB-2, p185erbB2, C-erbB-2, NEU proto-oncogene, tyrosine kinase-type cell surface receptor HER2, MLN 19, CD340 |
Gene, Accession # | ERBB2, Gene ID: 2064, NCBI: P04626.1, UniProt: P04626 |
Catalog # | 900-C01-B37 |
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Order / More Info | c-erbB2, extracellular Antibody from ROCKLAND IMMUNOCHEMICALS INC. |
Product Specific References | n/a |
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