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Antigenic Specificity | NAG-1, C-terminus |
Clone | 6D12.H10.E4 |
Host Species | Rat |
Reactive Species | human, mouse |
Isotype | IgG2a lambda |
Format | Protein G purified |
Size | 100 µg |
Concentration | 1.0 mg/mL |
Applications | ELISA, Western Blot. This Protein G purified antibody is suitable for ELISA and western blotting of human NAG-1 protein. For detection of NAG-1 in human serum, a sandwich ELISA is suggested using this antibody in combination with anti-NAG-1/GDF15 (N-terminal), H variant or D variant specific antibodies. Specific conditions for reactivity should be optimized by the end user. Expect bands in Western blots of native protein of approximately 13 and 26 kDa in size corresponding to NAG-1 monomer and dimer, respectively, using the appropriate cell lysate or extract. |
Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
Description | This product was purified from concentrated tissue culture supernatant Protein G chromatography. This antibody reacts with the C-terminus of endogenous NAG-1 protein from human tissues. A BLAST analysis suggests reactivity with NAG-1 from chimpanzee and macaque based on a 100% homology. Partial reactivity is expected against rat based on an 86% homology with the immunizing sequence. Cross-reactivity with NAG-1 from other sources has not been determined. |
Immunogen | This Protein-A purified antibody was prepared by repeated immunizations with an MBP-tagged recombinant protein produced in E.coli corresponding to full-length mouse NAG-1 protein. Cross reactivity to MBP was removed via cross-adsorption chromatography. |
Other Names | rat anti-NAG1 antibody, NAG-1, GDF15, MIC-1, nonsteroidal anti-inflammatory drug-activated gene, NSAID-activated gene 1 protein, growth differentiation factor 15, macrophage inhibitory compound 1, prostate-derived factor |
Gene, Accession # | GDF15, UniProt: Q99988 |
Catalog # | 200-501-B07 |
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Order / More Info | NAG-1, C-terminus Antibody from ROCKLAND IMMUNOCHEMICALS INC. |
Product Specific References | n/a |
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