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| Antigenic Specificity | Mesothelin, extracellular |
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| Clone | MN-1 |
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| Host Species | Mouse |
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| Reactive Species | human |
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| Isotype | IgG2a |
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| Format | Protein A purified |
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| Size | 100 µg |
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| Concentration | 1.0 mg/mL |
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| Applications | ELISA, Immunohistochemistry, Western Blot. This antibody has been tested for use in immunohistochemistry and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 40 kDa in size corresponding to mature mesothelin by western blotting in the appropriate cell lysate or extract. For immunohistochemistry, archival PEFF human tissues were deparaffinized followed by hydration. Antigen-retrieval is recommended. Block tissues with 1% BSA in PBS for 30 min at 23° C. Antibodies are diluted in 1% BSA and reacted with tissue for 60 min at room temperature. |
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| Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
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| Description | This antibody is directed against human mesothelin protein. This product was purified from tissue culture supernatant fluid by Protein A chromatography. Cross reactivity with homologues from other sources has not been tested. |
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| Immunogen | This antibody was produced in mesothelin-deficient mice by immunizations with plasmid cDNA encoding human MSLN full length protein followed by a single boost of a recombinant human mesothelin-Fc fusion protein. |
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| Other Names | mouse anti-Mesothelin Antibody, Mesothelian, MN, MB, Pre-pro-megakaryocyte-potentiating factor, CAK1 antigen |
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| Gene, Accession # | MSLN, Gene ID: 10232, NCBI: NP_005814, UniProt: Q13421 |
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| Catalog # | 200-301-A88 |
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| Price | |
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| Order / More Info | Mesothelin, extracellular Antibody from ROCKLAND IMMUNOCHEMICALS INC. |
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| Product Specific References | Onda, M., Willingham, M., Nagata, S., Bera, T.K., Beers, R., Ho, M., Hassan, R., Kreitman, R.J., Pastan, I. (2005) New monoclonal antibodies to mesothelin useful for immunohistochemistry, fluorescence-activated cell sorting, Western blotting, and ELISA. Clin Cancer Res. 11(16):5840-6.Ho, M., Bera, T.K., Willingham, M., Onda, M., Hassan, R., Fitzgerald, D. and Pastan, I. (2007) Mesothelin Expression in Human Lung Cancer. Clin Cancer Res 13:1571-5.Feng, M., Zhang, J., Anver, M., Hassan, R., & Ho, M. (2011). In vivo imaging of human malignant mesothelioma grown orthotopically in the peritoneal cavity of nude mice. Journal of Cancer, 2, 123.O'Shannessy, D. J., Somers, E. B., Palmer, L. M., Thiel, R. P., Oberoi, P., Heath, R., & Marcucci, L. (2013). Serum folate receptor alpha, mesothelin and megakaryocyte potentiating factor in ovarian cancer: association to disease stage and grade and comparison to CA125 and HE4. J Ovarian Res, 6(1), 29. |
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