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Antigenic Specificity | EphrinB phospho T317, internal |
Clone | polyclonal |
Host Species | Rabbit |
Reactive Species | chicken, rat |
Isotype | n/a |
Format | immunoaffinity purified |
Size | 100 µL |
Concentration | Titrated value sufficient to run approximately 10 mini blots. |
Applications | ELISA, Western Blot. Anti-EphrinB pT317 Antibody is suitable for use in ELISA and Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 46 kDa in size corresponding to EphrinB protein phosphorylated at Tyr317 in the appropriate cell lysate or extract. |
Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
Description | Anti-EphrinB pT317 antibody is directed against rat Ephrin. Anti-EphrinB pT317 antibody was prepared from monospecific antiserum by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum. This antibody is specific for phosphorylated EphrinB. Minimal reactivity occurs against non-phosphorylated EphrinB pT317. Reactivity is completely blocked by treatment with lambda-phosphatase. Reactivity against EphrinB occurs from chicken and rat sources. However, reactivity is also expected against the following species based on 100% sequence homology: bovine, human, mouse, Xenopus and zebra fish. |
Immunogen | Anti-EphrinB pT317 Antibody was produced in rabbit by repeated immunizations with synthetic phospho-peptide corresponding to amino acid residues surrounding Tyr317 conjugated to KLH. |
Other Names | Ephrin type-B receptor 2, EPH-like kinase 5, EK5 |
Gene, Accession # | EPHB2, Gene ID: 396513, NCBI: NP_996834.1, UniProt: P28693 |
Catalog # | 600-401-D36 |
Price | |
Order / More Info | EphrinB phospho T317, internal Antibody from ROCKLAND IMMUNOCHEMICALS INC. |
Product Specific References | n/a |