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Antigenic Specificity | Dynamin phospho S774, internal |
Clone | polyclonal |
Host Species | Sheep |
Reactive Species | rat |
Isotype | n/a |
Format | immunoaffinity purified |
Size | 100 µL |
Concentration | Titrated value sufficient to run approximately 10 mini blots. |
Applications | ELISA, Immunohistochemistry, IF Microscopy, Western Blot. Anti-Dynamin pS774 Antibody is suitable for use in ELISA, Western Blotting, IHC and IF. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 95 kDa in size corresponding to dynamin protein phosphorylated at Ser774 in the appropriate cell lysate or extract. Anti-Dynamin pS774 antibody labels the purified protein phosphorylated in vitro by cdk5 but not by PKC. Anti-Dynamin pS774 antibody does not cross react with other purified substrates of cdk5 (e.g. amphiphysin and synapsin). |
Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
Description | Anti-Dynamin pS774 antibody is directed against Dynamin. Anti-Dynamin pS774 antibody was prepared from monospecific antiserum by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum. This antibody is specific for phosphorylated Dynamin pS774. Minimal reactivity occurs against non-phosphorylated Dynamin pS774. Reactivity is completely blocked by treatment with lambda-phosphatase. Reactivity against Dynamin pS774 occurs from rat sources. However, reactivity is also expected against bovine, canine, chicken, human, mouse and NHP. |
Immunogen | Anti-Dynamin pS774 Antibody was produced in sheep by repeated immunizations with synthetic phospho-peptide corresponding to amino acid residues surrounding Ser774 conjugated to KLH. |
Other Names | sheep anti-Dynamin pS774 Antibody, Dynamin-1, B-dynamin, D100 |
Gene, Accession # | DNM1, Gene ID: 140694, NCBI: AAA16746.1, UniProt: P21575 |
Catalog # | 612-601-D32 |
Price | |
Order / More Info | Dynamin phospho S774, internal Antibody from ROCKLAND IMMUNOCHEMICALS INC. |
Product Specific References | n/a |
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