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Antigenic Specificity | DYKDDDDK Tag |
Clone | polyclonal |
Host Species | Rabbit |
Reactive Species | n/a |
Isotype | n/a |
Format | unconjugated |
Size | 25 µL |
Concentration | n/a |
Applications | Western Blotting (WB),ELISA,Immunoprecipitation (IP) |
Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
Description | Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the biochemical properties of the tagged protein. Most often, sequences encoding the epitope tag are included with the target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows Anti epitope tag antibodies to serve as universal detection reagents for any tag-containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. |
Immunogen | This antibody was purified from whole rabbit serum prepared by repeated immunizations with the Enterokinase Cleavage Site (ECS) peptide DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) conjugated to KLH using maleimide. Residues of glycine and cysteine were added to the carboxy terminal end to facilitate coupling. This antibody reacts with DYKDDDDK conjugated proteins.Immunogen Type: Peptide |
Other Names | n/a |
Gene, Accession # | n/a |
Catalog # | ABIN1043869 |
Price | |
Order / More Info | DYKDDDDK Tag Antibody from ANTIBODIES-ONLINE GmbH |
Product Specific References | PubMed: 8770418, 25386878 |