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Antigenic Specificity | ATP7b |
Clone | polyclonal |
Host Species | Rabbit |
Reactive Species | human, mouse, rat |
Isotype | n/a |
Format | immunogen affinity purified |
Size | 0.1 ml |
Concentration | 1 mg/ml |
Applications | Western Blot, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin. In WB this antibody recognizes a band at 165 kDa, representing ATP7b and also recognizes a faint non-specific band at 195 kDa. In IHC-P, cytoplasmic and golgi staining was observed in mouse liver tissue (1:100 dilution) and human ovarian carcinoma cells (1:500 dilution). However, please note we also tested this antibody with IHC-P in mouse intestines and kidney and observed unexpected strong nuclear signal with lighter cytoplasmic staining. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
Description | The ATP7b Antibody from Novus Biologicals is a rabbit polyclonal antibody to ATP7b. This antibody reacts with human, mouse, rat. The ATP7b Antibody has been validated for the following applications: Western Blot, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin. |
Immunogen | A synthetic peptide made to an internal sequence near the N-terminus of human ATP7b. [UniProt# P35670] |
Other Names | ATPase, Cu(2+)- transporting, beta polypeptide, ATPase, Cu++ transporting, beta polypeptide, Copper pump 2, copper-transporting ATPase 2, EC 3.6.3, EC 3.6.3.4, PWD, WC1, WD, Wilson disease-associated protein, WNDATPase, Cu++ transporting, beta polypeptide (Wilson disease) |
Gene, Accession # | ATP7B, Gene ID: 540, SwissProt: P35670 |
Catalog # | NB100-360 |
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Order / More Info | ATP7b Antibody from NOVUS BIOLOGICALS, LLC |
Product Specific References | PubMed: 17588765, 17978167, 17987273, 19470734, 20836889, 23221602, 23843956, 25801007, 29763364, 30351140 |
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