| 产品详情 |
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| Antigenic Specificity | CD44 |
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| Clone | MEM-263 |
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| Host Species | Mouse |
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| Reactive Species | human, porcine, canine |
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| Isotype | IgG1 |
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| Format | Protein A purified |
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| Size | 0.1 mg |
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| Concentration | 1.0 mg/ml |
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| Applications | Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin, CyTOF-ready. Western Blot - Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing SDS-PAGE sample buffer. Application note: Non-reducing conditions. SDS-PAGE (6% separating gel). Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 25481699). This antibody is CyTOF ready. |
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| Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
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| Description | The CD44 Antibody (MEM-263) from Novus Biologicals is a mouse monoclonal antibody to CD44. This antibody reacts with human, porcine, canine. The CD44 Antibody (MEM-263) has been validated for the following applications: Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin, CyTOF-ready. The antibody MEM-263 reacts with extracellular (N-terminal) domain of standard CD44 (Phagocyte glycoprotein 1), a 80-95 kDa transmembrane glycoprotein (hyaladherin family) present on the most of cells and tissues (leukocytes, endothelial cells, mesenchymal cells, etc |
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| Immunogen | COS-7 cells, African Green Monkey (NM_000610.3). |
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| Other Names | CD44 antigen, CD44 molecule (Indian blood group), CD44R, CDw44, cell surface glycoprotein CD44, chondroitin sulfate proteoglycan 8, CSPG8, ECMR-III, Epican, Extracellular matrix receptor III, GP90 lymphocyte homing/adhesion receptor, HCELL, hematopoietic cell E- and L-selectin ligand, Heparan sulfate proteoglycan, Hermes antigen, homing function and Indian blood group system, HUTCH-I, Hyaluronate receptor, IN, LHR, MC56, MDU2CD44 antigen (homing function and Indian blood group system), MDU3CDW44, MIC4MGC10468, Pgp1, PGP-1, PGP-I, Phagocytic glycoprotein 1, Phagocytic glycoprotein I |
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| Gene, Accession # | CD44, Gene ID: 960, Accession: P16070, SwissProt: P16070 |
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| Catalog # | NB500-481 |
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| Price | |
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| Order / More Info | CD44 Antibody from NOVUS BIOLOGICALS, LLC |
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| Product Specific References | PubMed: 21904548, 23528228, 25349438, 25481699, 30399175 |
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