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| Product Name | SEPX1, 1-116aa, Human, His tag, E.coli |
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| Description | Purity: > 90% by SDS - PAGE Format: Liquid. In 20 mM Tris-HCl Buffer (pH 7.5) containing 1 mM DTT, 0.1 mM PMSF, 2mM EDTA, 10% Glycerol . SEPX1, also known as Methionine sulfoxide reductase B1 (MSRB1), is a selenoprotein. Methionine sulfoxide reductases(MSRs) catalyze reduction of free and protein-bound methionine sulfoxides to corresponding methionines. The oxidation of methionine by ROS generates a diastereomeric mixture of methionine-S-sulfoxide (Met-S-SO) and methionine-R-sulfoxide (Met-R-SO). Two distinct enzyme families evolved for reduction of these sulfoxides, with methionine-S-sulfoxide reductase (MsrA) being stereospecific for Met-S-SO and methionine-R-sulfoxide reductase (MsrB) for Met-R-SO. In bacteria, the selenocystein (Sec/u) element is located immediately following the uGA codon within the reading frame for the selenoprotein so we mutated Sec-95 to Cys. Recombinant human SEPX1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography. |
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| Size | 250ug, 50ug, 10ug |
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| Concentration | n/a |
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| Applications | SDS-PAGE |
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| Other Names | Methionine-R-sulfoxide reductase B1, Selenoprotein X, HSPC270, MSRB1, SELR, SELX, MSRB1, Methionine-R-sulfoxide reductase B1, u95C |
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| Gene, Accession, CAS # | NP 057416 |
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| Catalog # | ATGP0735 |
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| Order / More Info | SEPX1, 1-116aa, Human, His tag, E.coli from ATGen |
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| Product Specific References | n/a |
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