Background

This human plasminogen total assay is for the quantitative determination of total plasminogen and plasmin in biological fluids.
Plasminogen is a single chain glycoprotein zymogen and is the precursor of the fibrinolytic enzyme plasmin. Plasminogen deficiencies are classified as hypoplasminogenemia (Type I) or dysplasminogenemia (Type 2) and are associated with decreased extracellular fibrin clearance leading to mucous membrane lesions and ligneous conjunctivitis (1).
 

Assay Principle

Human plasminogen will bind to the capture antibody coated on the microtiter plate. Plasminogen, plasmin, and plasmin in complex with antiplasmin will react with the antibody on the plate. After appropriate washing steps, polyclonal anti-human plasminogen primary antibody binds to the plasminogen. Excess antibody is washed away and bound polyclonal antibody is then reacted with the secondary antibody conjugated to horseradish peroxidase. Following an additional washing step, TMB substrate is used for color development at 450 nm. The amount of color development is directly proportional to the concentration of total plasminogen in the sample.
 

Typical Standard Curve

References

1.    Tefs, K., et al.; (2006) Blood; 108(9): 3021-3026
2.    Zolton, R.P., et al.; (1972) Clin. Chem.;18: 654-657