Description

Mouse Endocan is a proteoglycan composed of a 20 kDa mature polypeptide and a 30 kDa chondroitin / dermatan sulfate chain.

However, glycosylation is present only over a fraction of mouse endocan. It also comprises a large proportion of non-glycosylated endocan corresponding to the protein body of the molecule.

Chromatography by ion exchange on DEAE-Sepharose allows to separate the glycosylated fraction (retained on the column) and the non-glycosylated (not bound).

Significant biological activities identified: none

Caractéristiques

• Recombinant mouse Endocan expressed in a CHO cell line
• Product in bioreactor in fetal calf serum free medium
• Purified by ion exchange and immunoaffinity chromatographies
• PM: 20kDa Western blot
• Format: 50 µg
• Purity: > 90% polyacrylamide gel revealed by silver nitrate
• Storage at -80 ° C in phosphate buffer without preservatives
• Applications: bioassays

Documentation

• Datasheet
• Examples of protocols
• Yassine H, De Freitas Caires N, Depontieu F, Scherpereel A, Awad A, Tsicopoulos A, Leboeuf C, Janin A, Duez C, Grigoriu B, Lassalle P. The non glycanated endocan polypeptide slows tumor growth by inducing stromal inflammatory reaction. Oncotarget. 2015;6(5):2725-35
• Depontieu F, de Freitas Caires N, Gourcerol D, Giordano J, Grigoriu B, Delehedde M, Lassalle P. Development of monoclonal antibodies and ELISA specific for the mouse vascular endocan. J Immunol Methods. 2012;378(1-2):88-94

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