产品详情
Just Do It ELISA Kit H1 (human Endocan)
The JDI (Just Do It) ELISA Kit" H1 or JDIEK H1 is a ready-to-use ELISA kit which allows the quantification of the human Endocan in biological fluids (serum, plasma, etc.) and the human cell culture supernatants.
This reference ( LIK-1205) is only intended for research (RUO / research use only) . And not in diagnostic or therapeutic uses.
The JDIEK (Just Do It Elisa Kit) H1 is a sandwich ELISA (ie using two monoclonal anti-human Endocan antibodies) enables 40 samples to be analyzed in duplicate.
Simply have 100 µL of serum, plasma or human cell culture supernatant to perform this assay.
The JDIEK H1 (LIK-1205) contains all of the compounds to allow for the quantification of Endocan in biological fluids (serum, plasma, ascites fluid, vitreous humor, etc.) and in culture supernatants:
One 96-well plate pre-coated with capture Antibody,
two vials of lyophilized recombinant human Endocan for quantification (LIP-1101),
One vial with biotin-coupled, anti-human Endocan monoclonal antibody, said detection Antibody ,
One vial of enzyme reagent ,
One vial of ELISA buffer 20X,
One vial of substrate solution ,
One vial of the acid stop solution ,
Two adhesive films ELISA plate sealing .
The protocol (inserted into the kit and downloadable from our website) is easily achievable and provides highly reproducible results.
You can also buy independently of additional vials of lyophilized human Endocan (companion product: LIP-1101).
Detects the human and monkey Endocan
• Linearity: 0.3 to 5 ng / ml
• detection limit: 0.15 ng / ml
• Limit of quantification: 0.3 ng / ml
• Within run repeatability (% CV): 4.80%
• Inter Series reproducibility (% CV): 7.59%
• Interference: No interference observed with hemolyzed plasma, milky or jaundiced
Chang X, Bian Y, Wu Y, Huang Y, Wang K, Duan T. Endocan of the maternal placenta tissue is increased in pre-eclampsia. Int J Clin Exp Pathol. 2015 Nov 1;8(11):14733-40. eCollection 2015
Pauly D, Hamed S, Behnes M, Lepiorz D, Lang S, Akin I, Borggrefe M, Bertsch T, Hoffmann U. Endothelial cell-specific molecule-1/endocan: Diagnostic and prognostic value in patients suffering from severe sepsis and septic shock. J Crit Care. 2015 Sep 25.
Saldir M, Tunc T, Cekmez F, Cetinkaya M, Kalayci T, Fidanci K, Babacan O, Erdem G, Kocak N, Sari E, Akgul EO, Kul M. Endocan and Soluble Triggering Receptor Expressed on Myeloid Cells-1 as Novel Markers for Neonatal Sepsis. Pediatr Neonatol. 2015 Dec;56(6):415-21
Bălănescu P, Lădaru A, Bălănescu E, Voiosu T, Băicuş C, Dan GA. Endocan, Novel Potential Biomarker for Systemic Sclerosis: Results of a Pilot Study. J Clin Lab Anal. 2015 Sep 1.
Stoppelkamp S, Veseli K, Stang K, Schlensak C, Wendel HP, Walker T. Identification of Predictive Early Biomarkers for Sterile-SIRS after Cardiovascular Surgery. PLoS One. 2015 Aug 11;10(8):e0135527.
Rodrigues KF, Pietrani NT, Bosco AA, Sousa LP, Ferreira CN, Sandrim VC, Gomes KB. Endocan : a new biomarker associated with inflammation in type 2 diabetes mellitus? Diabetes Metab Res Rev. 2015 Jul;31(5):479-80.
Seo K, Kitazawa T, Yoshino Y, Koga I, Ota Y. Characteristics of serum endocan levels in infection. PLoS One. 2015 Apr 20;10(4):e0123358.
Palud A, Parmentier-Decrucq E, Pastre J, De Freitas Caires N, Lassalle P, Mathieu D. Evaluation of endothelial biomarkers as predictors of organ failures in septic shock patients. Cytokine. 2015 Jun;73(2):213-8.
Pawlak K, Mysliwiec M, Pawlak D. Endocan--the new endothelial activation marker independently associated with soluble endothelial adhesion molecules in uraemic patients with cardiovascular disease. Clin Biochem. 2015 Apr;48(6):425-30.
Elsheikh E, Younoszai Z, Otgonsuren M, Hunt S, Raybuck B, Younossi ZM. Markers of endothelial dysfunction in patients with non-alcoholic fatty liver disease and coronary artery disease. J Gastroenterol Hepatol. 2014;29(7):1528-34. No abstract available.
Cox LA, van Eijk LT, Ramakers BP, Dorresteijn MJ, Gerretsen J, Kox M, Pickkers P. Inflammation-induced increases in plasma endocan levels are associated with endothelial dysfunction in humans in vivo. Shock. 2015 Apr;43(4):322-6.
Lindås R, Tvedt TH, Hatfield KJ, Reikvam H, Bruserud O. >Preconditioning serum levels of endothelial cell-derived molecules and the risk of posttransplant complications in patients treated with allogeneic stem cell transplantation. J Transplant. 2014;2014:404096.
Mosevoll KA, Lindås R, Wendelbo O, Bruserud O, Reikvam H. Systemic levels of the endothelium-derived soluble adhesion molecules endocan and E-selectin in patients with suspected deep vein thrombosis. Springerplus. 2014 Sep 30;3:571.
Tang L, Zhao Y, Wang D, Deng W, Li C, Li Q, Huang S, Shu C. Endocan levels in peripheral blood predict outcomes of acute respiratory distress syndrome. Mediators Inflamm. 2014;2014:625180.
Abu El-Asrar AM, Nawaz MI, De Hertogh G, Al-Kharashi AS, Van den Eynde K, Mohammad G, Geboes K. The angiogenic biomarker endocan is upregulated in proliferative diabetic retinopathy and correlates with vascular endothelial growth factor. Curr Eye Res. 2015 Mar;40(3):321-31.
Ozaki K, Toshikuni N, George J, Minato T, Matsue Y, Arisawa T, Tsutsumi M. Serum endocan as a novel prognostic biomarker in patients with hepatocellular carcinoma. J Cancer. 2014 Mar 4;5(3):221-30.
Su YH, Shu KH, Hu CP, Cheng CH, Wu MJ, Yu TM, Chuang YW, Huang ST, Chen CH. Serum Endocan correlated with stage of chronic kidney disease and deterioration in renal transplant recipients. Transplant Proc. 2014;46(2):323-7.
Palmiere C, Augsburger M. Endocan measurement for the postmortem diagnosis of sepsis. Leg Med (Tokyo). 2014 Jan;16(1):1-7.
Kao SJ, Chuang CY, Tang CH, Lin CH, Bien MY, Yu MC, Bai KJ, Yang SF, Chien MH. Plasma endothelial cell-specific molecule-1 (ESM-1) in management of community-acquired pneumonia. Clin Chem Lab Med. 2014 Mar;52(3):445-51.
Oltean S, Pullerits R, Flodén A, Olausson M, Oltean M. Increased resistin in brain dead organ donors is associated with delayed graft function after kidney transplantation. J Transl Med. 2013 Sep 26;11:233. doi: 10.1186/1479-5876-11-233.
Nault JC, Guyot E, Laguillier C, Chevret S, Ganne-Carrie N, N'Kontchou G, Beaugrand M, Seror O, Trinchet JC, Coelho J, Lasalle P, Charnaux N, Delehedde M, Sutton A, Nahon P. Serum proteoglycans as prognostic biomarkers of hepatocellular carcinoma in patients with alcoholic cirrhosis. Cancer Epidemiol Biomarkers Prev. 2013 Aug;22(8):1343-52.
Schuitemaker J, Woudenberg J, Wijbenga G, Scherjon S, van Pampus M, Faas M. PP077. New prognostic marker for the risk to develop early-onset preeclampsia. Pregnancy Hypertens. 2013 Apr;3(2):95-6.
De Freitas Caires N, Legendre B, Parmentier E, Scherpereel A, Tsicopoulos A, Mathieu D, Lassalle P. Identification of a 14 kDa endocan fragment generated by cathepsin G, a novel circulating biomarker in patients with sepsis. J Pharm Biomed Anal. 2013 May 5;78-79:45-51.
Roudnicky F, Poyet C, Wild P, Krampitz S, Negrini F, Huggenberger R, Rogler A, Stöhr R, Hartmann A, Provenzano M, Otto VI, Detmar M. Endocan is upregulated on tumor vessels in invasive bladder cancer where it mediates VEGF-A-induced angiogenesis. Cancer Res. 2013 Feb 1;73(3):1097-106.
Check our pages Clinical areas and Endocan for more information.
Anti-endocan antibody mouse (N-Ter) 100 µg GGR222: LIA-0905
Anti- endocan antibody human/mouse/rat (C-Ter) 100 µg MEP14: LIA-1001
Anti-endocan antibody mouse/rat (Internal) 100 µg GGR237: LIA-1101
Standard mouse Endocan: LIP-1104
This reference can be also ordered in superior quantity (bulk). Do not hesitate to contact us for any questions and/or quotation request at This email address is being protected from spambots. You need JavaScript enabled to view it. .
