Description

Mouse Endocan is a proteoglycan composed of a 20 kDa mature polypeptide and a 30 kDa chondroitin / dermatan sulfate chain.

However, glycosylation is present only on a fraction of mouse endocan. It also comprises a large proportion of non-glycosylated endocan corresponding to the mature protein core of the molecule.

Chromatography by ion exchange on DEAE-Sepharose allows to separate the glycosylated (retained on the column) and the non-glycosylated fractions (not bound).

Key identified biological activities :

• protumoral

Characteristics

• Recombinant murine Endocan expressed in CHO cells
• Produced in bioreactor in fetal calf serum free medium
• Purified by ion exchange chromatography coupled to immunoaffinity chromatography
• PM: 50 kDa in western blot 
• Format: 50 µg
• Purity: > 90% polyacrylamide gel revealed by silver nitrate
• Storage at -80 ° C in phosphate buffer without preservatives
• Applications: bioassays

Documentation

• Datasheet
• Examples of protocols
• Yassine H, De Freitas Caires N, Depontieu F, Scherpereel A, Awad A, Tsicopoulos A, Leboeuf C, Janin A, Duez C, Grigoriu B, Lassalle P. The non glycanated endocan polypeptide slows tumor growth by inducing stromal inflammatory reaction. Oncotarget. 2015;6(5):2725-35
• Depontieu F, de Freitas Caires N, Gourcerol D, Giordano J, Grigoriu B, Delehedde M, Lassalle P. Development of monoclonal antibodies and ELISA specific for the mouse vascular endocan. J Immunol Methods. 2012;378(1-2):88-94

Visit our webpages Clinicals Areas and Endocan for more informations.

Complementary products

• Endomark® H1 : LIDM-1201
• Anticorps anti-endocan humain (N-Ter) MEP21 100 µg : LIA-0902
• Anticorps anti-endocan humain (C-Ter) MEP14 100 µg : LIA-1001
• Do It Yourself ELISA Kit H1 (Endocan humain) : LIK-1101