Description

Mutation of the single glycosylation Site (S137A) prevents the formation of the glycan chain endocan.

This mutation generates a non-glycosylated 20 kDa protein corresponding to the body of the molecule.

Key identified biological activities :

• Anti-tumor, 
• Pro-inflammatory.

Characteristics

• Recombinant human S137A Endocan expressed by a human cell line type HEK293
• Produced in bioreactor in fetal calf serum free medium
• Purified by ion exchange and  immunoaffinity chromatographies
• PM: 20kDa Western blot
• Format: 10 µg
• Purity: > 90% polyacrylamide gel revealed by silver nitrate
• Storage at -80 ° C in phosphate buffer without preservatives
• Applications: bioassays

Documentation

• Datasheet
• Examples of protocols
• Béchard D, Gentina T, Delehedde M, Scherpereel A, Lyon M, Aumercier M, Vazeux R, Richet C, Degand P, Jude B, Janin A, Fernig DG, Tonnel AB, Lassalle P. Endocan is a novel chondroitin sulfate/dermatan sulfate proteoglycan that promotes hepatocyte growth factor/scatter factor mitogenic activity. J Biol Chem. 2001;276(51):48341-9
• Adam E, Sarrazin S, Landolfi C, Motte V, Lortat-Jacob H, Lassalle P, Delehedde M. Efficient long-term and high-yielded production of a recombinant proteoglycan in eukaryotic HEK293 cells using a membrane-based bioreactor.Biochem Biophys Res Commun. 2008;369(2):297-302
• Yassine H, De Freitas Caires N, Depontieu F, Scherpereel A, Awad A, Tsicopoulos A, Leboeuf C, Janin A, Duez C, Grigoriu B, Lassalle P. The non glycanated endocan polypeptide slows tumor growth by inducing stromal inflammatory reaction. Oncotarget. 2015;6(5):2725-35

Visit our webpages Clinicals Areas and Endocan for more informations.

Complementary products

• Endomark® H1 : LIDM-1201
• Anticorps anti-endocan humain (N-Ter) MEP21 100 µg : LIA-0902
• Anticorps anti-endocan humain (C-Ter) MEP14 100 µg : LIA-1001
• Do It Yourself ELISA Kit H1 (Endocan humain) : LIK-1101